Curated Information
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Home > Curated Information Page > PubMed Id: 20015946
Dynia DW, Steinmetz AG, Kocinsky HS (2010) NHE3 function and phosphorylation are regulated by a calyculin A-sensitive phosphatase. Am J Physiol Renal Physiol 298, F745-53 20015946
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S552-p - NHE3 (rat)
Modsite: AEGERRGsLAFIRSP SwissProt Entrez-Gene
Orthologous residues
NHE3 (human): S555‑p, NHE3 (mouse): S550‑p, NHE3 (rat): S552‑p, NHE3 (rabbit): S554‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), OKP (epithelial), PS120 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, hamster, opossum
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP1CA (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
calyculin_A increase
IBMX, colforsin increase
okadaic_acid no change compared to control

S605-p - NHE3 (rat)
Modsite: SLEQRRRsIRDTEDM SwissProt Entrez-Gene
Orthologous residues
NHE3 (human): S607‑p, NHE3 (mouse): S603‑p, NHE3 (rat): S605‑p, NHE3 (rabbit): S607‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), OKP (epithelial), PS120 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, hamster, opossum
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP1CA (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
calyculin_A increase
IBMX, colforsin increase
okadaic_acid no change compared to control

S661-p - NHE3 (rat)
Modsite: TMRKRLEsFKSAKLG SwissProt Entrez-Gene
Orthologous residues
NHE3 (human): S663‑p, NHE3 (mouse): S659‑p, NHE3 (rat): S661‑p, NHE3 (rabbit): S663‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  COS (fibroblast), PS120 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, hamster

S716-p - NHE3 (rat)
Modsite: KEKDLELsEPEEATN SwissProt Entrez-Gene
Orthologous residues
NHE3 (human): S718‑p, NHE3 (mouse): S714‑p, NHE3 (rat): S716‑p, NHE3 (rabbit): S719‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  COS (fibroblast), PS120 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, hamster