Spengler ML, Kuropatwinski KK, Schumer M, Antoch MP (2009) A serine cluster mediates BMAL1-dependent CLOCK phosphorylation and degradation. Cell Cycle 8, 4138-46 19946213

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

Download

S427-p - CLOCK (human) ▲

Modsite: ALERFDHsPtPsASs SwissProt Entrez-Gene Orthologous residues CLOCK (human): S427‑p, CLOCK (mouse): S427‑p, CLOCK (rat): S427‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE GSK3B (human) pharmacological activator of upstream enzyme, transfection of dominant-negative enzyme, transfection of constitutively active enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes BMAL1 (human) increase LY294002 increase Downstream Regulation Effect of modification (function):  protein degradation Effect of modification (process):  transcription, altered

S431-p - CLOCK (human) ▲

Modsite: FDHsPtPsASsRSsR SwissProt Entrez-Gene Orthologous residues CLOCK (human): S431‑p, CLOCK (mouse): S431‑p, CLOCK (rat): S431‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes BMAL1 (human) increase LY294002 increase Downstream Regulation Effect of modification (function):  phosphorylation, protein degradation Effect of modification (process):  transcription, altered Comments:  primes phosphorylation of S427 by GSK3B

S434-p - CLOCK (human) ▲

Modsite: sPtPsASsRSsRKSS SwissProt Entrez-Gene Orthologous residues CLOCK (human): S434‑p, CLOCK (mouse): S434‑p, CLOCK (rat): S434‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes BMAL1 (human) increase Downstream Regulation Effect of modification (function):  protein degradation

S437-p - CLOCK (human) ▲

Modsite: PsASsRSsRKSSHtA SwissProt Entrez-Gene Orthologous residues CLOCK (human): S437‑p, CLOCK (mouse): S437‑p, CLOCK (rat): S437‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes BMAL1 (human) increase Downstream Regulation Effect of modification (function):  protein degradation