Curated Information
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Home > Curated Information Page > PubMed Id: 19822666
Densham RM, et al. (2009) MST kinases monitor actin cytoskeletal integrity and signal via c-Jun N-terminal kinase stress-activated kinase to regulate p21Waf1/Cip1 stability. Mol Cell Biol 29, 6380-90 19822666
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T57-p - p21Cip1 (human)
Modsite: NFDFVTEtPLEGDFA SwissProt Entrez-Gene
Orthologous residues
p21Cip1 (human): T57‑p, p21Cip1 (mouse): T56‑p, p21Cip1 (dog): T25‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE JNK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
botulinum_C3_toxin increase
MST1 (mouse) increase
MST2 (mouse) increase
Downstream Regulation
Effect of modification (function):  protein stabilization

S98-p - p21Cip1 (human)
Modsite: GGRRPGTsPALLQGT SwissProt Entrez-Gene
Orthologous residues
p21Cip1 (human): S98‑p, p21Cip1 (mouse): S96‑p, p21Cip1 (dog): S66‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse

S130-p - p21Cip1 (human)
Modsite: sGEQAEGsPGGPGDs SwissProt Entrez-Gene
Orthologous residues
p21Cip1 (human): S130‑p, p21Cip1 (mouse): S125‑p, p21Cip1 (dog): S98‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse

T145-p - p21Cip1 (human)
Modsite: QGRkRRQtsMTDFyH SwissProt Entrez-Gene
Orthologous residues
p21Cip1 (human): T145‑p, p21Cip1 (mouse): T140‑p, p21Cip1 (dog): T113‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse

S146-p - p21Cip1 (human)
Modsite: GRkRRQtsMTDFyHs SwissProt Entrez-Gene
Orthologous residues
p21Cip1 (human): S146‑p, p21Cip1 (mouse): S141‑p, p21Cip1 (dog): S114‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse

S63-p - Jun (mouse)
Modsite: kNsDLLtsPDVGLLK SwissProt Entrez-Gene
Orthologous residues
Jun (human): S63‑p, Jun (mouse): S63‑p, Jun (rat): S63‑p, Jun (cow): S63‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE JNK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JNK1 (mouse) activation of upstream enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
botulinum_C3_toxin increase
SP600125 botulinum_C3_toxin inhibit treatment-induced increase
SP600125 increase
MST1 (mouse) increase
SP600125 MST1 (mouse) inhibit treatment-induced increase
MST2 (mouse) increase
SP600125 MST2 (mouse) inhibit treatment-induced increase