Akita Y, et al. (2007) Protein kinase C epsilon phosphorylates keratin 8 at Ser8 and Ser23 in GH4C1 cells stimulated by thyrotropin-releasing hormone. FEBS J 274, 3270-85 17553064

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S9-p - K8 (mouse) ▲

Modsite: sIRVTQKsYkMstsG SwissProt Entrez-Gene Orthologous residues K8 (human): S9‑p, K8 iso2 (human): S37‑p, K8 (mouse): S9‑p, K8 (rat): S9‑p Characterization Methods used to characterize site in vivo:  2D analysis, immunoprecipitation, mass spectrometry, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  GH4C1 (pituitary cell) Cellular systems studied:  cell lines Species studied:  rat Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCE (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKCE (rat) activation of upstream enzyme, microscopy-colocalization, 2D analysis, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes TRH increase

S24-p - K8 (mouse) ▲

Modsite: PrAFssRsFtsGPGA SwissProt Entrez-Gene Orthologous residues K8 (human): S24‑p, K8 iso2 (human): S52‑p, K8 (mouse): S24‑p, K8 (rat): S24‑p Characterization Methods used to characterize site in vivo:  2D analysis, immunoprecipitation, mass spectrometry, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  GH4C1 (pituitary cell) Cellular systems studied:  cell lines Species studied:  rat Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCE (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKCE (rat) activation of upstream enzyme, microscopy-colocalization, 2D analysis, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes TRH increase

S47-p - K8 (mouse) ▲

Modsite: RVGsssssFRGSMGT SwissProt Entrez-Gene Orthologous residues K8 (human): ‑, K8 iso2 (human): ‑, K8 (mouse): S47‑p, K8 (rat): S47‑p Characterization Methods used to characterize site in vivo:  2D analysis, immunoprecipitation, mass spectrometry, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  GH4C1 (pituitary cell) Cellular systems studied:  cell lines Species studied:  rat Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKCE (human)