Curated Information
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Home > Curated Information Page > PubMed Id: 17579213
Giraud J, et al. (2007) Phosphorylation of Irs1 at SER-522 inhibits insulin signaling. Mol Endocrinol 21, 2294-302 17579213
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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T308-p - Akt1 (rat)
Modsite: KDGATMKtFCGTPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin insulin inhibit treatment-induced decrease
PD98059 insulin no change compared to control
rapamycin insulin no change compared to control
glucose_starvation no change compared to control
insulin glucose_starvation increase

T203-p - ERK1 (rat)
Modsite: HDHTGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin insulin inhibit treatment-induced increase
PD98059 insulin inhibit treatment-induced increase
rapamycin insulin no change compared to control
glucose_starvation no change compared to control
insulin glucose_starvation increase

Y205-p - ERK1 (rat)
Modsite: HTGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
glucose_starvation no change compared to control
insulin glucose_starvation increase
insulin increase
wortmannin insulin inhibit treatment-induced increase
PD98059 insulin inhibit treatment-induced increase
rapamycin insulin no change compared to control

T183-p - ERK2 (rat)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
glucose_starvation no change compared to control
insulin glucose_starvation increase
insulin increase
wortmannin insulin inhibit treatment-induced increase
PD98059 insulin inhibit treatment-induced increase
rapamycin insulin no change compared to control

Y185-p - ERK2 (rat)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
glucose_starvation no change compared to control
insulin glucose_starvation increase
insulin increase
wortmannin insulin inhibit treatment-induced increase
PD98059 insulin inhibit treatment-induced increase
rapamycin insulin no change compared to control

S302-p - IRS1 (rat)
Modsite: TRRSRTEsITATsPA SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S307‑p, IRS1 (mouse): S302‑p, IRS1 (rat): S302‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  rat

S522-p - IRS1 (rat)
Modsite: RFRKRTHsAGtsPTI SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S527‑p, IRS1 (mouse): S522‑p, IRS1 (rat): S522‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), CHO (fibroblast) [IRS1 (human)], L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (rat) siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin insulin inhibit treatment-induced decrease
PD98059 insulin no change compared to control
rapamycin insulin no change compared to control
glucose_starvation no change compared to control
insulin glucose_starvation increase
siRNA, insulin inhibit treatment-induced increase Akt siRNA

T389-p - p70S6K iso2 (rat)
Modsite: NQVFLGFtYVAPSVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin insulin inhibit treatment-induced increase
PD98059 insulin inhibit treatment-induced decrease
rapamycin insulin inhibit treatment-induced increase
glucose_starvation no change compared to control
insulin glucose_starvation increase