Curated Information
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Home > Curated Information Page > Cst Curation Set #8083
Tucker M (2009) CST Curation Set: 8083; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb Cat#: 10001, PTMScan(R) Phospho-Akt Substrate Motif (RXRXXS*/T*) Immunoaffinity Beads Cat#: 1979
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S1126-p - Afadin (mouse)
Modsite: sGKPRPKsEGFELyN SwissProt Entrez-Gene
Orthologous residues
Afadin (human): S1126‑p, Afadin iso1 (human): S1109‑p, Afadin iso2 (human): S1110‑p, Afadin iso3 (human): S1109‑p, Afadin iso5 (human): S1125‑p, Afadin (mouse): S1126‑p, Afadin iso2 (mouse): S1111‑p, Afadin (rat): S1133‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

T14-p - CDK1 (mouse)
Modsite: IEKIGEGtyGVVyKG SwissProt Entrez-Gene
Orthologous residues
CDK1 (human): T14‑p, CDK1 iso2 (human): T14‑p, CDK1 (mouse): T14‑p, CDK1 (rat): T14‑p, CDK1 (chicken): T14‑p, CDK1 (fruit fly): T14‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

Y15-p - CDK1 (mouse)
Modsite: EKIGEGtyGVVyKGR SwissProt Entrez-Gene
Orthologous residues
CDK1 (human): Y15‑p, CDK1 iso2 (human): Y15‑p, CDK1 (mouse): Y15‑p, CDK1 (rat): Y15‑p, CDK1 (chicken): Y15‑p, CDK1 (fruit fly): Y15‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

T14-p - CDK2 (mouse)
Modsite: VEKIGEGtyGVVyKA SwissProt Entrez-Gene
Orthologous residues
CDK2 (human): T14‑p, CDK2 iso2 (human): T14‑p, CDK2 (mouse): T14‑p, CDK2 (rat): T14‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

Y15-p - CDK2 (mouse)
Modsite: EKIGEGtyGVVyKAK SwissProt Entrez-Gene
Orthologous residues
CDK2 (human): Y15‑p, CDK2 iso2 (human): Y15‑p, CDK2 (mouse): Y15‑p, CDK2 (rat): Y15‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

T14-p - CDK3 (mouse)
Modsite: VEKIGEGtyGVVyKA SwissProt Entrez-Gene
Orthologous residues
CDK3 (human): T14‑p, CDK3 (mouse): T14‑p, CDK3 (cow): T14‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

Y15-p - CDK3 (mouse)
Modsite: EKIGEGtyGVVyKAR SwissProt Entrez-Gene
Orthologous residues
CDK3 (human): Y15‑p, CDK3 (mouse): Y15‑p, CDK3 (cow): Y15‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S451-p - CTSC (mouse)
Modsite: TDECAIEsIAVAAIP SwissProt Entrez-Gene
Orthologous residues
CTSC (human): S452‑p, CTSC (mouse): S451‑p, CTSC (rat): S451‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S161-p - EDC3 (mouse)
Modsite: SFRRRHNsWsSssRH SwissProt Entrez-Gene
Orthologous residues
EDC3 (human): S161‑p, EDC3 (mouse): S161‑p, EDC3 (rat): S160‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

T173-p - EDC3 (mouse)
Modsite: sRHPNQAtPKKSGLK SwissProt Entrez-Gene
Orthologous residues
EDC3 (human): T173‑p, EDC3 (mouse): T173‑p, EDC3 (rat): T172‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

T532-p - EPC1 (mouse)
Modsite: WRHFRPRtPsLPDSD SwissProt Entrez-Gene
Orthologous residues
EPC1 (human): T533‑p, EPC1 (mouse): T532‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S534-p - EPC1 (mouse)
Modsite: HFRPRtPsLPDSDSG SwissProt Entrez-Gene
Orthologous residues
EPC1 (human): S535‑p, EPC1 (mouse): S534‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S278-p - GSK3A (mouse)
Modsite: VRGEPNVsyICsRYY SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): S278‑p, GSK3A (mouse): S278‑p, GSK3A (rat): S278‑p, GSK3A (cow): S278‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

Y279-p - GSK3A (mouse)
Modsite: RGEPNVsyICsRYYR SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): Y279‑p, GSK3A (mouse): Y279‑p, GSK3A (rat): Y279‑p, GSK3A (cow): Y279‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S215-p - GSK3B (mouse)
Modsite: VRGEPNVsyICsRYY SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S215‑p, GSK3B iso2 (human): S215‑p, GSK3B (mouse): S215‑p, GSK3B (rat): S215‑p, GSK3B (rabbit): S215‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

Y216-p - GSK3B (mouse)
Modsite: RGEPNVsyICsRYYR SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): Y216‑p, GSK3B iso2 (human): Y216‑p, GSK3B (mouse): Y216‑p, GSK3B (rat): Y216‑p, GSK3B (rabbit): Y216‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S242-p - HMOX1 (mouse)
Modsite: sLRQRPAsLVQDTAP SwissProt Entrez-Gene
Orthologous residues
HMOX1 (human): N242‑p, HMOX1 (mouse): S242‑p, HMOX1 (rat): S242‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S32-p - K18 (mouse)
Modsite: QRVrPAssAAsVyAG SwissProt Entrez-Gene
Orthologous residues
K18 (human): S31‑p, K18 (mouse): S32‑p, K18 (rat): S32‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

T421-p - Lamin B2 (mouse)
Modsite: GKRRRLEtEDtsGsP SwissProt Entrez-Gene
Orthologous residues
Lamin B2 (human): V441‑p, Lamin B2 (mouse): T421‑p, Lamin B2 (rat): T441‑p, Lamin B2 (chicken): A420‑p, Lamin B2 (cow): V446‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S544-p - LARP (mouse)
Modsite: VKKRPRPsPARPKKP SwissProt Entrez-Gene
Orthologous residues
LARP (human): S567‑p, LARP iso3 (human): S490‑p, LARP (mouse): S544‑p, LARP (rat): S480‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S256-p - LARP7 (mouse)
Modsite: PRtAsEGsEAEtPEA SwissProt Entrez-Gene
Orthologous residues
LARP7 (human): S261‑p, LARP7 iso2 (human): , LARP7 iso3 (human): S268‑p, LARP7 (mouse): S256‑p, LARP7 (rat): S257‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

T260-p - LARP7 (mouse)
Modsite: sEGsEAEtPEAPKQP SwissProt Entrez-Gene
Orthologous residues
LARP7 (human): S265‑p, LARP7 iso2 (human): , LARP7 iso3 (human): S272‑p, LARP7 (mouse): T260‑p, LARP7 (rat): T261‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S132-p - LSD1 (mouse)
Modsite: DEsLANLsEDEyysE SwissProt Entrez-Gene
Orthologous residues
LSD1 (human): S131‑p, LSD1 iso2 (human): S131‑p, LSD1 (mouse): S132‑p, LSD1 iso2 (mouse): S132‑p, LSD1 (rat): S131‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S138-p - LSD1 (mouse)
Modsite: LsEDEyysEEERNAK SwissProt Entrez-Gene
Orthologous residues
LSD1 (human): S137‑p, LSD1 iso2 (human): S137‑p, LSD1 (mouse): S138‑p, LSD1 iso2 (mouse): S138‑p, LSD1 (rat): S137‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S391-p - MARK3 (mouse)
Modsite: LAKVRPNsDLsNsTG SwissProt Entrez-Gene
Orthologous residues
MARK3 (human): S391‑p, MARK3 iso3 (human): S391‑p, MARK3 iso6 (human): S375‑p, MARK3 iso7 (human): S398‑p, MARK3 (mouse): S391‑p, MARK3 (rat): S391‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S394-p - MARK3 (mouse)
Modsite: VRPNsDLsNsTGQsP SwissProt Entrez-Gene
Orthologous residues
MARK3 (human): N394‑p, MARK3 iso3 (human): N394‑p, MARK3 iso6 (human): N378‑p, MARK3 iso7 (human): N401‑p, MARK3 (mouse): S394‑p, MARK3 (rat): S394‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S400-p - MARK3 (mouse)
Modsite: LsNsTGQsPHHKGQR SwissProt Entrez-Gene
Orthologous residues
MARK3 (human): S400‑p, MARK3 iso3 (human): S400‑p, MARK3 iso6 (human): S384‑p, MARK3 iso7 (human): S407‑p, MARK3 (mouse): S400‑p, MARK3 (rat): S400‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S481-p - MLH1 (mouse)
Modsite: RKRHREDsDVEMVEN SwissProt Entrez-Gene
Orthologous residues
MLH1 (human): S477‑p, MLH1 iso2 (human): S236‑p, MLH1 (mouse): S481‑p, MLH1 (rat): S479‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

T19-p - MRLC1 (mouse)
Modsite: KKRPQRAtsNVFAMF SwissProt Entrez-Gene
Orthologous residues
MRLC1 (human): T19‑p, MRLC1 (mouse): T19‑p, MRLC1 (rat): T19‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S20-p - MRLC1 (mouse)
Modsite: KRPQRAtsNVFAMFD SwissProt Entrez-Gene
Orthologous residues
MRLC1 (human): S20‑p, MRLC1 (mouse): S20‑p, MRLC1 (rat): S20‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

T19-p - MRLC2 (mouse)
Modsite: KKRPQRAtsNVFAMF SwissProt Entrez-Gene
Orthologous residues
MRLC2 (human): T19‑p, MRLC2 (mouse): T19‑p, MRLC2 (rat): T19‑p, MRLC2 (cow): T19‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S20-p - MRLC2 (mouse)
Modsite: KRPQRAtsNVFAMFD SwissProt Entrez-Gene
Orthologous residues
MRLC2 (human): S20‑p, MRLC2 (mouse): S20‑p, MRLC2 (rat): S20‑p, MRLC2 (cow): S20‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

T19-p - MRLC3 (mouse)
Modsite: KKRPQRAtsNVFAMF SwissProt Entrez-Gene
Orthologous residues
MRLC3 (human): T18‑p, MRLC3 (mouse): T19‑p, MRLC3 (rat): T19‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S20-p - MRLC3 (mouse)
Modsite: KRPQRAtsNVFAMFD SwissProt Entrez-Gene
Orthologous residues
MRLC3 (human): S19‑p, MRLC3 (mouse): S20‑p, MRLC3 (rat): S20‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S1468-p - PDCD11 (mouse)
Modsite: GKRERQEsEsEQELV SwissProt Entrez-Gene
Orthologous residues
PDCD11 (human): S1476‑p, PDCD11 (mouse): S1468‑p, PDCD11 (rat): S1480‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

Y849-p - PRP4 (mouse)
Modsite: ADNDItPyLVsRFYR SwissProt Entrez-Gene
Orthologous residues
PRP4 (human): Y849‑p, PRP4 (mouse): Y849‑p, PRP4 (rat): Y849‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S1369-p - PRX (mouse)
Modsite: GFRVRLPsVGFSETA SwissProt Entrez-Gene
Orthologous residues
PRX (human): S1439‑p, PRX iso2 (human): , PRX iso3 (human): S1300‑p, PRX (mouse): S1369‑p, PRX (rat): S1361‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

T112-p - Pumilio-1 (mouse)
Modsite: TSKHRWPtGDNIHAE SwissProt Entrez-Gene
Orthologous residues
Pumilio‑1 (human): T112‑p, Pumilio‑1 iso2 (human): T112‑p, Pumilio‑1 iso3 (human): T112‑p, Pumilio‑1 iso4 (human): T148‑p, Pumilio‑1 (mouse): T112‑p, Pumilio‑1 (rat): T112‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S254-p - RCOR1 (mouse)
Modsite: QKREREEsEDELEEt SwissProt Entrez-Gene
Orthologous residues
RCOR1 (human): S260‑p, RCOR1 (mouse): S254‑p, RCOR1 (rat): S140‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S20-p - RRM2 (mouse)
Modsite: DQQQLQLsPLKRLTL SwissProt Entrez-Gene
Orthologous residues
RRM2 (human): S20‑p, RRM2 iso2 (human): S80‑p, RRM2 (mouse): S20‑p, RRM2 (rat): S20‑p, RRM2 (cow): S17‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

T931-p - SETD1A (mouse)
Modsite: EKRPRPstPAEEDED SwissProt Entrez-Gene
Orthologous residues
SETD1A (human): T916‑p, SETD1A (mouse): T931‑p, SETD1A (rat): T929‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S559-p - SPATA13 iso6 (mouse)
Modsite: RTRPRPLsDYGQLAG SwissProt Entrez-Gene
Orthologous residues
SPATA13 (human): , SPATA13 iso6 (human): S591‑p, SPATA13 (mouse): , SPATA13 iso6 (mouse): S559‑p, SPATA13 (rat): S562‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S234-p - SWT1 (mouse)
Modsite: ESGRKKIsFKIPKKS SwissProt Entrez-Gene
Orthologous residues
SWT1 (human): S233‑p, SWT1 (mouse): S234‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

T181-p - TMEM252 (mouse)
Modsite: ALQLTELtGR_____ SwissProt Entrez-Gene
Orthologous residues
TMEM252 (human): , TMEM252 (mouse): T181‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S154-p - treacle (mouse)
Modsite: VHLLsGKsPKKsAEP SwissProt Entrez-Gene
Orthologous residues
treacle (human): S156‑p, treacle iso2 (human): S156‑p, treacle iso3 (human): S156‑p, treacle iso4 (human): S156‑p, treacle iso6 (human): S156‑p, treacle (mouse): S154‑p, treacle iso3 (mouse): S154‑p, treacle (rat): S153‑p, treacle (cow): S159‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse

S57-p - YAF2 (mouse)
Modsite: TRKPRPVsQLVAQQV SwissProt Entrez-Gene
Orthologous residues
YAF2 (human): S57‑p, YAF2 iso3 (human): S15‑p, YAF2 (mouse): S57‑p, YAF2 (rat): S57‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  mouse