Curated Information
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Home > Curated Information Page > PubMed Id: 17287469
Witczak CA, Fujii N, Hirshman MF, Goodyear LJ (2007) Ca2+/calmodulin-dependent protein kinase kinase-alpha regulates skeletal muscle glucose uptake independent of AMP-activated protein kinase and Akt activation. Diabetes 56, 1403-9 17287469
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S212-p - ACC2 (mouse)
Modsite: PtMRPsMsGLHLVKR SwissProt Entrez-Gene
Orthologous residues
ACC2 (human): S222‑p, ACC2 (mouse): S212‑p, ACC2 (rat): S218‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal' [CAMKK1 (mouse)]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Skeletal muscle was transfected with constitutively active CAMKK in vivo.
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
muscle contraction increase
STO-609 muscle contraction no effect upon treatment-induced increase

T308-p - Akt1 (mouse)
Modsite: KDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal' [CAMKK1 (mouse)]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Skeletal muscle was transfected with constitutively active CAMKK in vivo.
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CAMKK1 (mouse) no change compared to control
insulin CAMKK1 (mouse) increase

T172-p - AMPKA2 (mouse)
Modsite: sDGEFLRtsCGsPNY SwissProt Entrez-Gene
Orthologous residues
AMPKA2 (human): T172‑p, AMPKA2 (mouse): T172‑p, AMPKA2 (rat): T172‑p, AMPKA2 (pig): T172‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal' [CAMKK1 (mouse)]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Skeletal muscle was transfected with constitutively active CAMKK in vivo.
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CAMKK1 (mouse) increase constitutively active CAMKK1
insulin CAMKK1 (mouse) no effect upon treatment-induced increase
muscle contraction increase muscle from wild-type mice
STO-609 muscle contraction no effect upon treatment-induced increase

T649-p - AS160 (mouse)
Modsite: QFRRRAHtFsHPPSS SwissProt Entrez-Gene
Orthologous residues
AS160 (human): T642‑p, AS160 iso2 (human): T642‑p, AS160 iso3 (human): T642‑p, AS160 (mouse): T649‑p, AS160 iso2 (mouse): T649‑p, AS160 (rat): T651‑p, AS160 (cow): T644‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal' [CAMKK1 (mouse)]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Skeletal muscle was transfected with constitutively active CAMKK in vivo.
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
CAMKK1 (mouse) no change compared to control
insulin CAMKK1 (mouse) increase

T177-p - CAMK1A (mouse)
Modsite: DPGSVLstACGtPGY SwissProt Entrez-Gene
Orthologous residues
CAMK1A (human): T177‑p, CAMK1A (mouse): T177‑p, CAMK1A (rat): T177‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal' [CAMKK1 (mouse)]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  Skeletal muscle was transfected with constitutively active CAMKK in vivo.
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CAMKK1 (mouse) transfection of constitutively active enzyme