Curated Information
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Home > Curated Information Page > PubMed Id: 10601309
Feng J, et al. (1999) Inhibitory phosphorylation site for Rho-associated kinase on smooth muscle myosin phosphatase. J Biol Chem 274, 37385-90 10601309
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T694-p - MYPT1 (mouse)
Modsite: ARQSRRstQGVTLTD SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): T696‑p, MYPT1 iso2 (human): T696‑p, MYPT1 iso3 (human): T640‑p, MYPT1 iso4 (human): T637‑p, MYPT1 (mouse): T694‑p, MYPT1 (rat): T697‑p, MYPT1 (pig): T696‑p, MYPT1 (chicken): T695‑p, MYPT1 (cow): T696‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  Swiss 3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ROCK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPA increase
Y27632 LPA inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, inhibited

T852-p - MYPT1 (mouse)
Modsite: PREKRRstGVSFWtQ SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): T853‑p, MYPT1 iso2 (human): T853‑p, MYPT1 iso3 (human): T797‑p, MYPT1 iso4 (human): T794‑p, MYPT1 (mouse): T852‑p, MYPT1 (rat): T855‑p, MYPT1 (pig): , MYPT1 (chicken): T850‑p, MYPT1 (cow): T851‑p