El Kouhen R, et al. (2001) Phosphorylation of Ser363, Thr370, and Ser375 residues within the carboxyl tail differentially regulates mu-opioid receptor internalization. J Biol Chem 276, 12774-80 11278523

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S363-p - MOR-1 (rat) ▲

Modsite: stIEQQNsTRVRQNt SwissProt Entrez-Gene Orthologous residues MOR‑1 (human): S365‑p, MOR‑1 iso5 (human): S365‑p, MOR‑1 (mouse): S363‑p, MOR‑1 (rat): S363‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes DAMGO increase Downstream Regulation Effect of modification (function):  receptor internalization, altered Comments:  Mutation of Ser363 and Thr370 to Ala accelerated MOR internalization kinetics.

T370-p - MOR-1 (rat) ▲

Modsite: sTRVRQNtREHPsTA SwissProt Entrez-Gene Orthologous residues MOR‑1 (human): T372‑p, MOR‑1 iso5 (human): T372‑p, MOR‑1 (mouse): T370‑p, MOR‑1 (rat): T370‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes DAMGO increase Downstream Regulation Effect of modification (function):  receptor internalization, altered Comments:  Mutation of Ser363 and Thr370 to Ala accelerated MOR internalization kinetics.

S375-p - MOR-1 (rat) ▲

Modsite: QNtREHPsTANTVDR SwissProt Entrez-Gene Orthologous residues MOR‑1 (human): S377‑p, MOR‑1 iso5 (human): S377‑p, MOR‑1 (mouse): S375‑p, MOR‑1 (rat): S375‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes DAMGO increase Downstream Regulation Effect of modification (function):  receptor internalization, altered Comments:  Mutation of Ser375 to Ala reduced the rate and extent of receptor internalization.