Curated Information
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Home > Curated Information Page > PubMed Id: 17537547
Zhang CE, et al. (2008) Homocysteine induces tau phosphorylation by inactivating protein phosphatase 2A in rat hippocampus. Neurobiol Aging 29, 1654-65 17537547
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S9-p - GSK3B (rat)
Modsite: SGRPRTTsFAESCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
homocysteine no change compared to control
vitamin_B12, folic acid no change compared to control

Y307-p - PPP2CA (rat)
Modsite: VTRRTPDyFl_____ SwissProt Entrez-Gene
Orthologous residues
PPP2CA (human): Y307‑p, PPP2CA iso2 (human): Y253‑p, PPP2CA (mouse): Y307‑p, PPP2CA (rat): Y307‑p, PPP2CA (pig): Y307‑p, PPP2CA (cow): Y307‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
homocysteine increase
vitamin_B12, folic acid homocysteine inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PPP2CB (rat) Disrupts enzymatic activity, inhibited co-immunoprecipitation
Comments:  Demethylated, phosphorylated PPP2CA decreased binding to PPP2CB.

L309-me - PPP2CA (rat)
Modsite: RRTPDyFl_______ SwissProt Entrez-Gene
Orthologous residues
PPP2CA (human): L309‑me, PPP2CA iso2 (human): L255‑me, PPP2CA (mouse): L309‑me, PPP2CA (rat): L309‑me, PPP2CA (pig): L309‑me, PPP2CA (cow): L309‑me
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
homocysteine decrease
vitamin_B12, folic acid inhibit treatment-induced decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PPP2CB (rat) Disrupts enzymatic activity, inhibited co-immunoprecipitation
Comments:  Demethylated, phosphorylated PPP2CA decreased binding to PPP2CB.

S510-p - Tau (rat)
Modsite: GERSGyssPGsPGtP SwissProt Entrez-Gene
Orthologous residues
Tau (human): S516‑p, Tau iso2 (human): S141‑p, Tau iso3 (human): S105‑p, Tau iso4 (human): S170‑p, Tau iso5 (human): S199‑p, Tau iso6 (human): S141‑p, Tau iso7 (human): S170‑p, Tau iso8 (human): S199‑p, Tau (mouse): S491‑p, Tau iso3 (mouse): S188‑p, Tau iso7 (mouse): S148‑p, Tau (rat): S510‑p, Tau (cow): S206‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP2CA (rat) microscopy-colocalization
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
homocysteine increase
vitamin_B12, folic acid homocysteine inhibit treatment-induced increase

S513-p - Tau (rat)
Modsite: SGyssPGsPGtPGsR SwissProt Entrez-Gene
Orthologous residues
Tau (human): S519‑p, Tau iso2 (human): S144‑p, Tau iso3 (human): S108‑p, Tau iso4 (human): S173‑p, Tau iso5 (human): S202‑p, Tau iso6 (human): S144‑p, Tau iso7 (human): S173‑p, Tau iso8 (human): S202‑p, Tau (mouse): S494‑p, Tau iso3 (mouse): S191‑p, Tau iso7 (mouse): S151‑p, Tau (rat): S513‑p, Tau (cow): S209‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP2CA (rat) microscopy-colocalization
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
homocysteine increase
vitamin_B12, folic acid homocysteine inhibit treatment-induced increase

T516-p - Tau (rat)
Modsite: ssPGsPGtPGsRSRt SwissProt Entrez-Gene
Orthologous residues
Tau (human): T522‑p, Tau iso2 (human): T147‑p, Tau iso3 (human): T111‑p, Tau iso4 (human): T176‑p, Tau iso5 (human): T205‑p, Tau iso6 (human): T147‑p, Tau iso7 (human): T176‑p, Tau iso8 (human): T205‑p, Tau (mouse): T497‑p, Tau iso3 (mouse): T194‑p, Tau iso7 (mouse): T154‑p, Tau (rat): T516‑p, Tau (cow): T212‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP2CA (rat) microscopy-colocalization
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
homocysteine increase
vitamin_B12, folic acid homocysteine inhibit treatment-induced increase

S525-p - Tau (rat)
Modsite: GsRSRtPsLPtPPTR SwissProt Entrez-Gene
Orthologous residues
Tau (human): S531‑p, Tau iso2 (human): S156‑p, Tau iso3 (human): S120‑p, Tau iso4 (human): S185‑p, Tau iso5 (human): S214‑p, Tau iso6 (human): S156‑p, Tau iso7 (human): S185‑p, Tau iso8 (human): S214‑p, Tau (mouse): S506‑p, Tau iso3 (mouse): S203‑p, Tau iso7 (mouse): S163‑p, Tau (rat): S525‑p, Tau (cow): S221‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP2CA (rat) microscopy-colocalization
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
homocysteine increase
vitamin_B12, folic acid homocysteine inhibit treatment-induced increase

T542-p - Tau (rat)
Modsite: KKVAVVRtPPKsPSA SwissProt Entrez-Gene
Orthologous residues
Tau (human): T548‑p, Tau iso2 (human): T173‑p, Tau iso3 (human): T137‑p, Tau iso4 (human): T202‑p, Tau iso5 (human): T231‑p, Tau iso6 (human): T173‑p, Tau iso7 (human): T202‑p, Tau iso8 (human): T231‑p, Tau (mouse): T523‑p, Tau iso3 (mouse): T220‑p, Tau iso7 (mouse): T180‑p, Tau (rat): T542‑p, Tau (cow): T238‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP2CA (rat) microscopy-colocalization
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
homocysteine increase
vitamin_B12, folic acid homocysteine inhibit treatment-induced increase

S573-p - Tau (rat)
Modsite: NVRSKIGsTENLKHQ SwissProt Entrez-Gene
Orthologous residues
Tau (human): S579‑p, Tau iso2 (human): S204‑p, Tau iso3 (human): S168‑p, Tau iso4 (human): S233‑p, Tau iso5 (human): S262‑p, Tau iso6 (human): S204‑p, Tau iso7 (human): S233‑p, Tau iso8 (human): S262‑p, Tau (mouse): S554‑p, Tau iso3 (mouse): S251‑p, Tau iso7 (mouse): S211‑p, Tau (rat): S573‑p, Tau (cow): S269‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP2CA (rat) microscopy-colocalization
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
homocysteine increase
vitamin_B12, folic acid homocysteine inhibit treatment-induced increase

S707-p - Tau (rat)
Modsite: GAEIVyKsPVVsGDt SwissProt Entrez-Gene
Orthologous residues
Tau (human): S713‑p, Tau iso2 (human): S307‑p, Tau iso3 (human): S271‑p, Tau iso4 (human): S336‑p, Tau iso5 (human): S365‑p, Tau iso6 (human): S338‑p, Tau iso7 (human): S367‑p, Tau iso8 (human): S396‑p, Tau (mouse): S688‑p, Tau iso3 (mouse): S385‑p, Tau iso7 (mouse): S345‑p, Tau (rat): S707‑p, Tau (cow): S403‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP2CA (rat) microscopy-colocalization
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
homocysteine increase
vitamin_B12, folic acid homocysteine inhibit treatment-induced increase

S715-p - Tau (rat)
Modsite: PVVsGDtsPRHLSNV SwissProt Entrez-Gene
Orthologous residues
Tau (human): S721‑p, Tau iso2 (human): S315‑p, Tau iso3 (human): S279‑p, Tau iso4 (human): S344‑p, Tau iso5 (human): S373‑p, Tau iso6 (human): S346‑p, Tau iso7 (human): S375‑p, Tau iso8 (human): S404‑p, Tau (mouse): S696‑p, Tau iso3 (mouse): S393‑p, Tau iso7 (mouse): S353‑p, Tau (rat): S715‑p, Tau (cow): S411‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP2CA (rat) microscopy-colocalization
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
homocysteine increase
vitamin_B12, folic acid homocysteine inhibit treatment-induced increase