Curated Information
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Home > Curated Information Page > Cst Curation Set #710
Rush J (2005) CST Curation Set: 710; Year: 2005; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)XX[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) Akt Substrate Antibody Cat#: 9611
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S292-p - AML1 (human)
Modsite: TLSAELSsRLstAPD SwissProt Entrez-Gene
Orthologous residues
AML1 (human): S292‑p, AML1 iso2 (human): S292‑p, AML1 iso7 (human): , AML1 iso8 (human): S319‑p, AML1 iso10 (human): S307‑p, AML1 (mouse): S292‑p, AML1 (rat): S291‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T296-p - AML1 (human)
Modsite: ELSsRLstAPDLTAF SwissProt Entrez-Gene
Orthologous residues
AML1 (human): T296‑p, AML1 iso2 (human): T296‑p, AML1 iso7 (human): , AML1 iso8 (human): T323‑p, AML1 iso10 (human): T311‑p, AML1 (mouse): T296‑p, AML1 (rat): T295‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T323-p - AML1 iso8 (human)
Modsite: ELSSRLStAPDLTAF SwissProt Entrez-Gene
Orthologous residues
AML1 (human): T296‑p, AML1 iso2 (human): T296‑p, AML1 iso7 (human): , AML1 iso8 (human): T323‑p, AML1 iso10 (human): T311‑p, AML1 (mouse): T296‑p, AML1 (rat): T295‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T1023-p - ANKRD27 (human)
Modsite: RRMLRRHtVEDAVVs SwissProt Entrez-Gene
Orthologous residues
ANKRD27 (human): T1023‑p, ANKRD27 (mouse): T1022‑p, ANKRD27 (rat): T1028‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

S702-p - ARHGAP17 (human)
Modsite: LsAPRRYsssLsPIQ SwissProt Entrez-Gene
Orthologous residues
ARHGAP17 (human): S702‑p, ARHGAP17 iso2 (human): S624‑p, ARHGAP17 iso5 (human): S702‑p, ARHGAP17 (mouse): S698‑p, ARHGAP17 iso2 (mouse): S620‑p, ARHGAP17 (rat): S710‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

S703-p - ARHGAP17 (human)
Modsite: sAPRRYsssLsPIQA SwissProt Entrez-Gene
Orthologous residues
ARHGAP17 (human): S703‑p, ARHGAP17 iso2 (human): S625‑p, ARHGAP17 iso5 (human): S703‑p, ARHGAP17 (mouse): S699‑p, ARHGAP17 iso2 (mouse): S621‑p, ARHGAP17 (rat): S711‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

S184-p - BORCS6 (human)
Modsite: GGAGSSGsAEsGAGG SwissProt Entrez-Gene
Orthologous residues
BORCS6 (human): S184‑p, BORCS6 (mouse): S189‑p, BORCS6 (rat): S189‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T232-p - CD7 (human)
Modsite: MsHsRCNtLssPNQy SwissProt Entrez-Gene
Orthologous residues
CD7 (human): T232‑p, CD7 (mouse): T202‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T339-p - CDP138 (human)
Modsite: KALLRQQtQsALEQR SwissProt Entrez-Gene
Orthologous residues
CDP138 (human): T339‑p, CDP138 iso2 (human): T330‑p, CDP138 iso4 (human): T330‑p, CDP138 (mouse): T339‑p, CDP138 iso2 (mouse): T330‑p, CDP138 (rat): T339‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T302-p - FTSJ1 (human)
Modsite: CPISRVDtFPQPLAA SwissProt Entrez-Gene
Orthologous residues
FTSJ1 (human): T302‑p, FTSJ1 iso2 (human): T300‑p, FTSJ1 (mouse): K300‑p, FTSJ1 (rat): K302‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T285-p - GTF2E1 (human)
Modsite: PIWLREStVQGAYGs SwissProt Entrez-Gene
Orthologous residues
GTF2E1 (human): T285‑p, GTF2E1 (mouse): T285‑p, GTF2E1 (rat): T171‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

S246-p - hnRNP E1 (human)
Modsite: NQVARQQsHFAMMHG SwissProt Entrez-Gene
Orthologous residues
hnRNP E1 (human): S246‑p, hnRNP E1 (mouse): S246‑p, hnRNP E1 (rat): S403‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

S1999-p - HUWE1 (human)
Modsite: RSLtRQssDFDTQSG SwissProt Entrez-Gene
Orthologous residues
HUWE1 (human): S1999‑p, HUWE1 iso2 (human): S1999‑p, HUWE1 (mouse): S1999‑p, HUWE1 (rat): S731‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T129-p - LSM1 (human)
Modsite: LsIPRADtLDEy___ SwissProt Entrez-Gene
Orthologous residues
LSM1 (human): T129‑p, LSM1 (mouse): T129‑p, LSM1 (rat): T129‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T447-p - MDC1 (human)
Modsite: VLLQRsQtttERDsD SwissProt Entrez-Gene
Orthologous residues
MDC1 (human): T447‑p, MDC1 iso2 (human): T447‑p, MDC1 (mouse): S436‑p, MDC1 (rat): S432‑p, MDC1 (cow): A279‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T448-p - MDC1 (human)
Modsite: LLQRsQtttERDsDt SwissProt Entrez-Gene
Orthologous residues
MDC1 (human): T448‑p, MDC1 iso2 (human): T448‑p, MDC1 (mouse): A437‑p, MDC1 (rat): A433‑p, MDC1 (cow): S280‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T449-p - MDC1 (human)
Modsite: LQRsQtttERDsDtD SwissProt Entrez-Gene
Orthologous residues
MDC1 (human): T449‑p, MDC1 iso2 (human): T449‑p, MDC1 (mouse): S438‑p, MDC1 (rat): S434‑p, MDC1 (cow): A281‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T507-p - PKCD (human)
Modsite: FGEsRAstFCGtPDy SwissProt Entrez-Gene
Orthologous residues
PKCD (human): T507‑p, PKCD iso2 (human): T538‑p, PKCD (mouse): T505‑p, PKCD iso2 (mouse): T531‑p, PKCD (rat): T505‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T514-p - Pumilio-1 (human)
Modsite: GASQRPLtPNQNQQG SwissProt Entrez-Gene
Orthologous residues
Pumilio‑1 (human): T514‑p, Pumilio‑1 iso2 (human): T515‑p, Pumilio‑1 iso3 (human): T514‑p, Pumilio‑1 iso4 (human): T455‑p, Pumilio‑1 (mouse): T515‑p, Pumilio‑1 (rat): T515‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T396-p - Pumilio-2 (human)
Modsite: GAGQRPLtPNQGQQG SwissProt Entrez-Gene
Orthologous residues
Pumilio‑2 (human): T396‑p, Pumilio‑2 iso2 (human): T396‑p, Pumilio‑2 iso3 (human): T396‑p, Pumilio‑2 iso4 (human): T340‑p, Pumilio‑2 (mouse): T395‑p, Pumilio‑2 iso2 (mouse): T395‑p, Pumilio‑2 (rat): T395‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

S1383-p - RPAP1 (human)
Modsite: HyLQRLtstVLQNGV SwissProt Entrez-Gene
Orthologous residues
RPAP1 (human): S1383‑p, RPAP1 (mouse): T1399‑p, RPAP1 iso3 (mouse): T1434‑p, RPAP1 (rat): T1390‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

S319-p - SCAMP2 (human)
Modsite: RTFHRAAssAAQGAF SwissProt Entrez-Gene
Orthologous residues
SCAMP2 (human): S319‑p, SCAMP2 (mouse): S319‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T124-p - SUN2 (human)
Modsite: GLVGRKAtEDFLGSS SwissProt Entrez-Gene
Orthologous residues
SUN2 (human): T124‑p, SUN2 iso2 (human): T124‑p, SUN2 (mouse): S135‑p, SUN2 (rat): S134‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

S236-p - TNRC15 (human)
Modsite: GERWRPHsPDGPRsA SwissProt Entrez-Gene
Orthologous residues
TNRC15 (human): S236‑p, TNRC15 iso3 (human): S258‑p, TNRC15 iso4 (human): S236‑p, TNRC15 (mouse): S237‑p, TNRC15 iso3 (mouse): S186‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

S242-p - TNRC15 (human)
Modsite: HsPDGPRsAGWREHM SwissProt Entrez-Gene
Orthologous residues
TNRC15 (human): S242‑p, TNRC15 iso3 (human): S264‑p, TNRC15 iso4 (human): , TNRC15 (mouse): S243‑p, TNRC15 iso3 (mouse):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T82-p - TUBA1B (human)
Modsite: IDEVRtGtyRQLFHP SwissProt Entrez-Gene
Orthologous residues
TUBA1B (human): T82‑p, TUBA1B (mouse): T82‑p, TUBA1B (rat): T82‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T82-p - TUBA1C (human)
Modsite: IDEVRtGtyRQLFHP SwissProt Entrez-Gene
Orthologous residues
TUBA1C (human): T82‑p, TUBA1C (mouse): T82‑p, TUBA1C (rat): T82‑p, TUBA1C (cow): T82‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T489-p - UBXN4 (human)
Modsite: GKIyRLRtQDDGEDE SwissProt Entrez-Gene
Orthologous residues
UBXN4 (human): T489‑p, UBXN4 (mouse): T487‑p, UBXN4 (rat): T487‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human

T149-p - USP25 (human)
Modsite: ACLKRtPtEVWRDSR SwissProt Entrez-Gene
Orthologous residues
USP25 (human): T149‑p, USP25 iso1 (human): T149‑p, USP25 iso3 (human): T149‑p, USP25 (mouse): I149‑p, USP25 (rat): I150‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human