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Home > Curated Information Page > PubMed Id: 17569658
Rybin VO, et al. (2007) Protein kinase Cepsilon (PKCepsilon) and Src control PKCdelta activation loop phosphorylation in cardiomyocytes. J Biol Chem 282, 23631-8 17569658
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S744-p - PRKD1 (mouse)
Modsite: ARIIGEksFRRsVVG SwissProt Entrez-Gene
Orthologous residues
PRKD1 (human): S738‑p, PRKD1 (mouse): S744‑p, PRKD1 (rat): S744‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  cardiac
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCE (mouse) phospho-antibody
Comments:  overexpression of PKCE increases T505-p.
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester, H2O2, norepinephrine increase
GF109203X H2O2, norepinephrine, phorbol_ester inhibit treatment-induced decrease
Go_6976 H2O2, norepinephrine, phorbol_ester no change compared to control
LY294002 H2O2, norepinephrine, phorbol_ester no change compared to control

S748-p - PRKD1 (mouse)
Modsite: GEksFRRsVVGtPAY SwissProt Entrez-Gene
Orthologous residues
PRKD1 (human): S742‑p, PRKD1 (mouse): S748‑p, PRKD1 (rat): S748‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  cardiac
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCE (mouse) phospho-antibody
Comments:  overexpression of PKCE increases T505-p.
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester, H2O2, norepinephrine increase
GF109203X H2O2, norepinephrine, phorbol_ester inhibit treatment-induced decrease
Go_6976 H2O2, norepinephrine, phorbol_ester no change compared to control
LY294002 H2O2, norepinephrine, phorbol_ester no change compared to control

Y438-p - Src iso2 (mouse)
Modsite: TAPEAALyGRFTIKS SwissProt Entrez-Gene
Orthologous residues
Src (human): Y439‑p, Src iso2 (human): Y445‑p, Src (mouse): Y444‑p, Src iso2 (mouse): Y438‑p, Src (rat): Y439‑p, Src (chicken): Y436‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  cardiac
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 PKCE (mouse) augment treatment-induced increase PKCE increases T311-p without activating Src.

T308-p - Akt1 (rat)
Modsite: KDGATMKtFCGTPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  cardiac
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCE (mouse) phospho-antibody
Comments:  overexpression of PKCE increases T505-p.

S473-p - Akt1 (rat)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  cardiac
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCE (mouse) phospho-antibody
Comments:  overexpression of PKCE increases T505-p.

Y311-p - PKCD (rat)
Modsite: TPETVGIyQGFEKKT SwissProt Entrez-Gene
Orthologous residues
PKCD (human): Y313‑p, PKCD iso2 (human): Y313‑p, PKCD (mouse): Y311‑p, PKCD iso2 (mouse): Y311‑p, PKCD (rat): Y311‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  cardiac, MEF (fibroblast) [PKCE (mouse)], SYF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  mouse, rat
Comments:  PKCE expression increases PKCD T505-p and Y311-p.
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Src (rat)
Comments:  Src enhances in vitro PKCD autophosphorylation.
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 PKCE (mouse) augment treatment-induced increase PKCE increases T311-p without activating Src.
phorbol_ester PKCE (mouse) augment treatment-induced increase PKCE overexpression increases Y311 and T505 in Src+ cells but not in cells that lac Src, Yes and Fyn.

Y332-p - PKCD (rat)
Modsite: IPDNNGTyGKIWEGS SwissProt Entrez-Gene
Orthologous residues
PKCD (human): Y334‑p, PKCD iso2 (human): Y365‑p, PKCD (mouse): Y332‑p, PKCD iso2 (mouse): Y358‑p, PKCD (rat): Y332‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  cardiac, SYF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  mouse, rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Src (rat)
Comments:  Src enhances in vitro PKCD autophosphorylation.

T505-p - PKCD (rat)
Modsite: FGENRAstFCGTPDy SwissProt Entrez-Gene
Orthologous residues
PKCD (human): T507‑p, PKCD iso2 (human): T538‑p, PKCD (mouse): T505‑p, PKCD iso2 (mouse): T531‑p, PKCD (rat): T505‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  cardiac, MEF (fibroblast) [PKCE (mouse)], SYF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  mouse, rat
Comments:  PKCE expression increases PKCD T505-p and Y311-p.
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCE (mouse) phospho-antibody
KINASE PKCE (mouse) transfection of dominant-negative enzyme, transfection of wild-type enzyme
Comments:  overexpression of PKCE increases T505-p.
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester, H2O2, norepinephrine increase
GF109203X H2O2, norepinephrine, phorbol_ester inhibit treatment-induced decrease
Go_6976 H2O2, norepinephrine, phorbol_ester no change compared to control
LY294002 H2O2, norepinephrine, phorbol_ester no change compared to control
phorbol_ester PKCE (mouse) augment treatment-induced increase PKCE overexpression increases T505-p without increasing partitioning to membranes.
H2O2 PKCE (mouse) augment treatment-induced increase PKCE increases T311-p without activating Src.
UCN-01 PKCD (rat) inhibit treatment-induced decrease
phorbol_ester PKCE (mouse) augment treatment-induced increase PKCE overexpression increases Y311 and T505 in Src+ cells but not in cells that lac Src, Yes and Fyn.