Curated Information
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Home > Curated Information Page > PubMed Id: 17478428
Mu JJ, et al. (2007) A proteomic analysis of ataxia telangiectasia-mutated (ATM)/ATM-Rad3-related (ATR) substrates identifies the ubiquitin-proteasome system as a regulator for DNA damage checkpoints. J Biol Chem 282, 17330-4 17478428
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S26-p - BAT3 (human)
Modsite: VLVktLDsQTRTFIV SwissProt Entrez-Gene
Orthologous residues
BAT3 (human): S26‑p, BAT3 iso2 (human): S26‑p, BAT3 iso3 (human): S26‑p, BAT3 iso5 (human): S26‑p, BAT3 (mouse): S26‑p, BAT3 (rat): S26‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S218-p - BAT3 (human)
Modsite: PEPVALSsQTSEPVE SwissProt Entrez-Gene
Orthologous residues
BAT3 (human): S218‑p, BAT3 iso2 (human): S212‑p, BAT3 iso3 (human): S212‑p, BAT3 iso5 (human): S212‑p, BAT3 (mouse): S213‑p, BAT3 (rat): S212‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S556-p - FANCI (human)
Modsite: VLGSLSSsQCsQSLS SwissProt Entrez-Gene
Orthologous residues
FANCI (human): S556‑p, FANCI iso1 (human): S556‑p, FANCI (mouse): S555‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S559-p - FANCI (human)
Modsite: SLSSsQCsQSLSVsQ SwissProt Entrez-Gene
Orthologous residues
FANCI (human): S559‑p, FANCI iso1 (human): S559‑p, FANCI (mouse): T558‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S730-p - FANCI (human)
Modsite: LDksADFsQsTSIGI SwissProt Entrez-Gene
Orthologous residues
FANCI (human): S730‑p, FANCI iso1 (human): S730‑p, FANCI (mouse): S729‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S972-p - FANCI (human)
Modsite: SLLNLLSsQEEDFNS SwissProt Entrez-Gene
Orthologous residues
FANCI (human): S972‑p, FANCI iso1 (human): S912‑p, FANCI (mouse): S968‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S1121-p - FANCI (human)
Modsite: TLSEEAssQATLPNQ SwissProt Entrez-Gene
Orthologous residues
FANCI (human): S1121‑p, FANCI iso1 (human): S1061‑p, FANCI (mouse): S1122‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S927-p - HUWE1 (human)
Modsite: ISVNQWGsQLGLSVL SwissProt Entrez-Gene
Orthologous residues
HUWE1 (human): S927‑p, HUWE1 iso2 (human): S927‑p, HUWE1 (mouse): S927‑p, HUWE1 (rat):
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S2339-p - HUWE1 (human)
Modsite: GQPEVLSsQEMQVEN SwissProt Entrez-Gene
Orthologous residues
HUWE1 (human): S2339‑p, HUWE1 iso2 (human): S2339‑p, HUWE1 (mouse): S2339‑p, HUWE1 (rat): S1072‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S2636-p - HUWE1 (human)
Modsite: HDQsTAtsQAGTLSS SwissProt Entrez-Gene
Orthologous residues
HUWE1 (human): S2636‑p, HUWE1 iso2 (human): S2636‑p, HUWE1 (mouse): S2636‑p, HUWE1 (rat): S1369‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S3377-p - HUWE1 (human)
Modsite: kACsPCSsQSSSSGI SwissProt Entrez-Gene
Orthologous residues
HUWE1 (human): S3377‑p, HUWE1 iso2 (human): S3361‑p, HUWE1 (mouse): S3378‑p, HUWE1 (rat): S2111‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S3695-p - HUWE1 (human)
Modsite: ENVVIVAsQkRPLGG SwissProt Entrez-Gene
Orthologous residues
HUWE1 (human): S3695‑p, HUWE1 iso2 (human): S3679‑p, HUWE1 (mouse): S3696‑p, HUWE1 (rat): S2429‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S3797-p - HUWE1 (human)
Modsite: QAAtsESsQSEAsVR SwissProt Entrez-Gene
Orthologous residues
HUWE1 (human): S3797‑p, HUWE1 iso2 (human): S3781‑p, HUWE1 (mouse): S3798‑p, HUWE1 (rat): S2531‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S816-p - MED1 (human)
Modsite: DSSSSGHsQSTLFDS SwissProt Entrez-Gene
Orthologous residues
MED1 (human): S816‑p, MED1 (mouse): S816‑p, MED1 (rat): S801‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S872-p - MED1 (human)
Modsite: FNPDLLNsQsQSGFG SwissProt Entrez-Gene
Orthologous residues
MED1 (human): S872‑p, MED1 (mouse): S872‑p, MED1 (rat): S857‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S874-p - MED1 (human)
Modsite: PDLLNsQsQSGFGEE SwissProt Entrez-Gene
Orthologous residues
MED1 (human): S874‑p, MED1 (mouse): S874‑p, MED1 (rat): S859‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S887-p - MED1 (human)
Modsite: EEYFDESsQSGDNDD SwissProt Entrez-Gene
Orthologous residues
MED1 (human): S887‑p, MED1 (mouse): S887‑p, MED1 (rat): S872‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S953-p - MED1 (human)
Modsite: LMEHHsGsQGPLLTT SwissProt Entrez-Gene
Orthologous residues
MED1 (human): S953‑p, MED1 (mouse): S953‑p, MED1 (rat): S938‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S1134-p - MED1 (human)
Modsite: LsSSMyssQGSSGSs SwissProt Entrez-Gene
Orthologous residues
MED1 (human): S1134‑p, MED1 (mouse): S1136‑p, MED1 (rat): S1120‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S1141-p - MED1 (human)
Modsite: sQGSSGSsQSKNSsQ SwissProt Entrez-Gene
Orthologous residues
MED1 (human): S1141‑p, MED1 (mouse): S1143‑p, MED1 (rat): S1127‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S1147-p - MED1 (human)
Modsite: SsQSKNSsQsGGKPG SwissProt Entrez-Gene
Orthologous residues
MED1 (human): S1147‑p, MED1 (mouse): S1149‑p, MED1 (rat): S1133‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S403-p - RAD18 (human)
Modsite: TSVTNHFsQSKLDsP SwissProt Entrez-Gene
Orthologous residues
RAD18 (human): S403‑p, RAD18 (mouse): P442‑p, RAD18 (rat): P406‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S46-p - RFWD3 (human)
Modsite: VPADVVSsQGVPSIL SwissProt Entrez-Gene
Orthologous residues
RFWD3 (human): S46‑p, RFWD3 (mouse): S59‑p, RFWD3 (rat): S52‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S63-p - RFWD3 (human)
Modsite: APAEVISsQATPPLL SwissProt Entrez-Gene
Orthologous residues
RFWD3 (human): S63‑p, RFWD3 (mouse): S75‑p, RFWD3 (rat): S68‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S1851-p - RIF1 (human)
Modsite: sEAMsLEsQEsPNEN SwissProt Entrez-Gene
Orthologous residues
RIF1 (human): S1851‑p, RIF1 iso2  (human): S1851‑p, RIF1 (mouse): I1819‑p, RIF1 (rat): I1677‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S522-p - RNF20 (human)
Modsite: sGsALLQsQsstEDP SwissProt Entrez-Gene
Orthologous residues
RNF20 (human): S522‑p, RNF20 (mouse): S522‑p, RNF20 (rat): S522‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S545-p - RNF20 (human)
Modsite: PDsEDLSsQSsAsKA SwissProt Entrez-Gene
Orthologous residues
RNF20 (human): S545‑p, RNF20 (mouse): T545‑p, RNF20 (rat): T545‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S819-p - RNF40 (human)
Modsite: EQVLGLksQVDAQLL SwissProt Entrez-Gene
Orthologous residues
RNF40 (human): S819‑p, RNF40 iso3 (human): S819‑p, RNF40 (mouse): S819‑p, RNF40 (rat): S820‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S853-p - RNF40 (human)
Modsite: EkELTLRsQALELNK SwissProt Entrez-Gene
Orthologous residues
RNF40 (human): S853‑p, RNF40 iso3 (human): , RNF40 (mouse): S853‑p, RNF40 (rat): S854‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S1138-p - SLX4 (human)
Modsite: PDHsSSRsQKSSSKL SwissProt Entrez-Gene
Orthologous residues
SLX4 (human): S1138‑p, SLX4 iso2 (human): S826‑p, SLX4 (mouse): A943‑p, SLX4 (rat): A949‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S1271-p - SLX4 (human)
Modsite: sRSRDCSsQtQISSL SwissProt Entrez-Gene
Orthologous residues
SLX4 (human): S1271‑p, SLX4 iso2 (human): S959‑p, SLX4 (mouse): S1073‑p, SLX4 (rat): S1080‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S1631-p - SLX4 (human)
Modsite: PHCQTLAsQTYKPSR SwissProt Entrez-Gene
Orthologous residues
SLX4 (human): S1631‑p, SLX4 iso2 (human): S1319‑p, SLX4 (mouse): T1405‑p, SLX4 (rat): T1418‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S1716-p - SLX4 (human)
Modsite: GSDSSLSsQSSSSCE SwissProt Entrez-Gene
Orthologous residues
SLX4 (human): S1716‑p, SLX4 iso2 (human): S1404‑p, SLX4 (mouse): S1447‑p, SLX4 (rat): S1460‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S359-p - TAO3 (human)
Modsite: SMSVSTGsQSSSVNS SwissProt Entrez-Gene
Orthologous residues
TAO3 (human): S359‑p, TAO3 (mouse): S359‑p, TAO3 (rat): S359‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S551-p - TAO3 (human)
Modsite: DLTTFLEsQKKQYKI SwissProt Entrez-Gene
Orthologous residues
TAO3 (human): S551‑p, TAO3 (mouse): S551‑p, TAO3 (rat): S551‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S81-p - TDP1 (human)
Modsite: PKRQKsGsQEDLGWC SwissProt Entrez-Gene
Orthologous residues
TDP1 (human): S81‑p, TDP1 (mouse): S81‑p, TDP1 (rat): S81‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S365-p - TDP1 (human)
Modsite: TPGRFQGsQkDNWGH SwissProt Entrez-Gene
Orthologous residues
TDP1 (human): S365‑p, TDP1 (mouse): S366‑p, TDP1 (rat): S366‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S563-p - TDP1 (human)
Modsite: KQKFFAGsQEPMATF SwissProt Entrez-Gene
Orthologous residues
TDP1 (human): S563‑p, TDP1 (mouse): S564‑p, TDP1 (rat): S564‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S174-p - UBR5 (human)
Modsite: rGsGLLGsQPQPVIP SwissProt Entrez-Gene
Orthologous residues
UBR5 (human): S174‑p, UBR5 (mouse): S174‑p, UBR5 (rat): S164‑p, UBR5 (cow): S174‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S191-p - UBR5 (human)
Modsite: VIPEELIsQAQVVLQ SwissProt Entrez-Gene
Orthologous residues
UBR5 (human): S191‑p, UBR5 (mouse): S191‑p, UBR5 (rat): S181‑p, UBR5 (cow): S191‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S1532-p - UBR5 (human)
Modsite: SDQSSSSsQSQSSYI SwissProt Entrez-Gene
Orthologous residues
UBR5 (human): S1532‑p, UBR5 (mouse): S1526‑p, UBR5 (rat): S1516‑p, UBR5 (cow): S1532‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S1679-p - UBR5 (human)
Modsite: SDSDSSSsQSDDIEQ SwissProt Entrez-Gene
Orthologous residues
UBR5 (human): S1679‑p, UBR5 (mouse): S1673‑p, UBR5 (rat): S1663‑p, UBR5 (cow): S1679‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S2586-p - UBR5 (human)
Modsite: LRQLILAsQSSDADA SwissProt Entrez-Gene
Orthologous residues
UBR5 (human): S2586‑p, UBR5 (mouse): S2579‑p, UBR5 (rat): S2569‑p, UBR5 (cow): S2585‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S678-p - USP15 (human)
Modsite: LPSENENsQSEDSVG SwissProt Entrez-Gene
Orthologous residues
USP15 (human): S678‑p, USP15 iso2 (human): S649‑p, USP15 iso4 (human): , USP15 (mouse): S678‑p, USP15 iso2 (mouse): S650‑p, USP15 (rat): S649‑p, USP15 iso2 (rat): S678‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S495-p - USP28 (human)
Modsite: STSTEsSsQDVESTF SwissProt Entrez-Gene
Orthologous residues
USP28 (human): S495‑p, USP28 iso2 (human): S495‑p, USP28 iso3 (human): S495‑p, USP28 (mouse): S496‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S714-p - USP28 (human)
Modsite: ESSTNSSsQDYSTsQ SwissProt Entrez-Gene
Orthologous residues
USP28 (human): S714‑p, USP28 iso2 (human): S714‑p, USP28 iso3 (human): , USP28 (mouse): S720‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S720-p - USP28 (human)
Modsite: SsQDYSTsQEPSVAS SwissProt Entrez-Gene
Orthologous residues
USP28 (human): S720‑p, USP28 iso2 (human): S720‑p, USP28 iso3 (human): , USP28 (mouse): S726‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S718-p - USP34 (human)
Modsite: ATHIAQGsQESCITR SwissProt Entrez-Gene
Orthologous residues
USP34 (human): S718‑p, USP34 iso2 (human): S567‑p, USP34 (mouse): S744‑p, USP34 (rat): S748‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S1751-p - USP34 (human)
Modsite: NIHIkDAsQTTLLDL SwissProt Entrez-Gene
Orthologous residues
USP34 (human): S1751‑p, USP34 iso2 (human): S1600‑p, USP34 (mouse): S1788‑p, USP34 (rat): S1792‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S326-p - VCP (human)
Modsite: EVERRIVsQLLtLMD SwissProt Entrez-Gene
Orthologous residues
VCP (human): S326‑p, VCP (mouse): S326‑p, VCP (rat): S326‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase

S115-p - ZBTB2 (human)
Modsite: IQEASLAsQGAFSHP SwissProt Entrez-Gene
Orthologous residues
ZBTB2 (human): S115‑p, ZBTB2 (mouse): S115‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ATM (human) activation of upstream enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
hydroxyurea increase