Curated Information
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Home > Curated Information Page > PubMed Id: 11853558
Sapkota GP, et al. (2002) Identification and characterization of four novel phosphorylation sites (Ser31, Ser325, Thr336 and Thr366) on LKB1/STK11, the protein kinase mutated in Peutz-Jeghers cancer syndrome. Biochem J 362, 481-90 11853558
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S31-p - LKB1 (mouse)
Modsite: TFIHRIDstEVIyQP SwissProt Entrez-Gene
Orthologous residues
LKB1 (human): S31‑p, LKB1 iso2 (human): S31‑p, LKB1 (mouse): S31‑p, LKB1 (rat): S31‑p, LKB1 (pig):
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S325-p - LKB1 (mouse)
Modsite: ALVPIPPsPDTKDRW SwissProt Entrez-Gene
Orthologous residues
LKB1 (human): S325‑p, LKB1 iso2 (human): S325‑p, LKB1 (mouse): S325‑p, LKB1 (rat): S325‑p, LKB1 (pig): S231‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

T336-p - LKB1 (mouse)
Modsite: KDRWRSMtVVPYLED SwissProt Entrez-Gene
Orthologous residues
LKB1 (human): T336‑p, LKB1 iso2 (human): T336‑p, LKB1 (mouse): T336‑p, LKB1 (rat): T336‑p, LKB1 (pig): T242‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phospho-antibody, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE LKB1 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LKB1 (mouse) transfection of inactive enzyme, transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  activity, inhibited
Effect of modification (process):  cell growth, altered

T366-p - LKB1 (mouse)
Modsite: IEDGIIytQDFTVPG SwissProt Entrez-Gene
Orthologous residues
LKB1 (human): T363‑p, LKB1 iso2 (human): T363‑p, LKB1 (mouse): T366‑p, LKB1 (rat): T366‑p, LKB1 (pig): T271‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phospho-antibody, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE LKB1 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LKB1 (mouse) transfection of inactive enzyme, transfection of wild-type enzyme

S431-p - LKB1 (mouse)
Modsite: sNKIRRLsACKQQ__ SwissProt Entrez-Gene
Orthologous residues
LKB1 (human): S428‑p, LKB1 iso2 (human): , LKB1 (mouse): S431‑p, LKB1 (rat): S431‑p, LKB1 (pig): S339‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human