Curated Information
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Home > Curated Information Page > PubMed Id: 28614711
Goodell DJ, et al. (2017) DAPK1 Mediates LTD by Making CaMKII/GluN2B Binding LTP Specific. Cell Rep 19, 2231-2243 28614711
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T286-p - CAMK2A (human)
Modsite: sCMHRQEtVDCLKKF SwissProt Entrez-Gene
Orthologous residues
CAMK2A (human): T286‑p, CAMK2A iso2 (human): T286‑p, CAMK2A (mouse): T286‑p, CAMK2A iso2 (mouse): , CAMK2A (rat): T286‑p

S308-p - DAPK1 (human)
Modsite: ARKKWkQsVRLISLC SwissProt Entrez-Gene
Orthologous residues
DAPK1 (human): S308‑p, DAPK1 iso3 (human): S308‑p, DAPK1 (mouse): S308‑p, DAPK1 (rat): S308‑p
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
NMDAR2B (human) Disrupts in vitro, microscopy-colocalization
CAMK2A (human) Induces in vitro

S1303-p - NMDAR2B (human)
Modsite: NKLRRQHsyDtFVDL SwissProt Entrez-Gene
Orthologous residues
NMDAR2B (human): S1303‑p, NMDAR2B (mouse): S1303‑p, NMDAR2B (rat): S1303‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  'brain, hippocampus', 293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE DAPK1 (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DAPK1 (human) Induces in vitro, microscopy-colocalization
CAMK2A (human) Disrupts in vitro
CAMK2A (human) Induces in vitro

S308-p - DAPK1 (mouse)
Modsite: ARKKWKQsVRLISLC SwissProt Entrez-Gene
Orthologous residues
DAPK1 (human): S308‑p, DAPK1 iso3 (human): S308‑p, DAPK1 (mouse): S308‑p, DAPK1 (rat): S308‑p
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
NMDAR2B (human) Disrupts in vitro, microscopy-colocalization

S863-p - GluR1 (mouse)
Modsite: tstLPRNsGAGASGG SwissProt Entrez-Gene
Orthologous residues
GluR1 (human): S863‑p, GluR1 iso6 (human): S873‑p, GluR1 (mouse): S863‑p, GluR1 (rat): S863‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
long-term depression increase

S1303-p - NMDAR2B (mouse)
Modsite: NKLRRQHsyDtFVDL SwissProt Entrez-Gene
Orthologous residues
NMDAR2B (human): S1303‑p, NMDAR2B (mouse): S1303‑p, NMDAR2B (rat): S1303‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
long-term depression increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DAPK1 (human) Induces in vitro, microscopy-colocalization