Dogan T, et al. (2017) Role of the E3 ubiquitin ligase RNF157 as a novel downstream effector linking PI3K and MAPK signaling pathways to the cell cycle. J Biol Chem 292, 14311-14324 28655764

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S660-p - RNF157 (human) ▲

Modsite: NAQRRRLssssLEDS SwissProt Entrez-Gene Orthologous residues RNF157 (human): S660‑p, RNF157 (mouse): ‑, RNF157 iso2 (mouse): S661‑p, RNF157 (rat): S662‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, phospho-antibody, western blotting Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  A2058 (keratinocyte), MEL624 (melanocyte) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes CDK2 (human) increase Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation, ubiquitination Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CDH1 (human) Induces co-immunoprecipitation

S661-p - RNF157 (human) ▲

Modsite: AQRRRLssssLEDSE SwissProt Entrez-Gene Orthologous residues RNF157 (human): S661‑p, RNF157 (mouse): ‑, RNF157 iso2 (mouse): S662‑p, RNF157 (rat): P663‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, phospho-antibody, western blotting Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  A2058 (keratinocyte), MEL624 (melanocyte) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes CDK2 (human) increase Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation, ubiquitination Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CDH1 (human) Induces co-immunoprecipitation

S662-p - RNF157 (human) ▲

Modsite: QRRRLssssLEDSET SwissProt Entrez-Gene Orthologous residues RNF157 (human): S662‑p, RNF157 (mouse): ‑, RNF157 iso2 (mouse): S663‑p, RNF157 (rat): S664‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, phospho-antibody, western blotting Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  A2058 (keratinocyte), MEL624 (melanocyte) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes CDK2 (human) increase Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation, ubiquitination Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CDH1 (human) Induces co-immunoprecipitation

S663-p - RNF157 (human) ▲

Modsite: RRRLssssLEDSETR SwissProt Entrez-Gene Orthologous residues RNF157 (human): S663‑p, RNF157 (mouse): ‑, RNF157 iso2 (mouse): S664‑p, RNF157 (rat): S665‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, phospho-antibody, western blotting Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  A2058 (keratinocyte), MEL624 (melanocyte) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes CDK2 (human) increase Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation, ubiquitination Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CDH1 (human) Induces co-immunoprecipitation