Curated Information
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Home > Curated Information Page > PubMed Id: 28591578
Gheghiani L, et al. (2017) PLK1 Activation in Late G2 Sets Up Commitment to Mitosis. Cell Rep 19, 2060-2073 28591578
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S154-p - CCNA2 (human)
Modsite: PMDGSFEsPHTMDMS SwissProt Entrez-Gene
Orthologous residues
CCNA2 (human): S154‑p, CCNA2 (mouse): S145‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MK1775 increase

S126-p - CCNB1 (human)
Modsite: PILVDTAsPsPMETs SwissProt Entrez-Gene
Orthologous residues
CCNB1 (human): S126‑p, CCNB1 (mouse): S123‑p, CCNB1 (rat): S116‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

S75-p - CDC25C (human)
Modsite: PkRCLDLsNLSSGEI SwissProt Entrez-Gene
Orthologous residues
CDC25C (human): S75‑p, CDC25C iso3 (human): , CDC25C (mouse): S74‑p, CDC25C (frog): S75‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PLK1 (human) phospho-antibody, co-immunoprecipitation

Y15-p - CDK1 (human)
Modsite: EkIGEGtyGVVykGR SwissProt Entrez-Gene
Orthologous residues
CDK1 (human): Y15‑p, CDK1 iso2 (human): Y15‑p, CDK1 (mouse): Y15‑p, CDK1 (rat): Y15‑p, CDK1 (chicken): Y15‑p, CDK1 (fruit fly): Y15‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MK1775 decrease

T210-p - PLK1 (human)
Modsite: YDGERKktLCGtPNy SwissProt Entrez-Gene
Orthologous residues
PLK1 (human): T210‑p, PLK1 (mouse): T210‑p, PLK1 (rat): T210‑p, PLK1 (frog): T201‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human