Gheghiani L, et al. (2017) PLK1 Activation in Late G2 Sets Up Commitment to Mitosis. Cell Rep 19, 2060-2073 28591578

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S154-p - CCNA2 (human) ▲

Modsite: PMDGSFEsPHTMDMS SwissProt Entrez-Gene Orthologous residues CCNA2 (human): S154‑p, CCNA2 (mouse): S145‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes MK1775 increase

S126-p - CCNB1 (human) ▲
Modsite: PILVDTAsPsPMETs SwissProt Entrez-Gene Orthologous residues CCNB1 (human): S126‑p, CCNB1 (mouse): S123‑p, CCNB1 (rat): S116‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human

S75-p - CDC25C (human) ▲

Modsite: PkRCLDLsNLSSGEI SwissProt Entrez-Gene Orthologous residues CDC25C (human): S75‑p, CDC25C iso3 (human): ‑, CDC25C (mouse): S74‑p, CDC25C (frog): S75‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PLK1 (human) phospho-antibody, co-immunoprecipitation

Y15-p - CDK1 (human) ▲

Modsite: EkIGEGtyGVVykGR SwissProt Entrez-Gene Orthologous residues CDK1 (human): Y15‑p, CDK1 iso2 (human): Y15‑p, CDK1 (mouse): Y15‑p, CDK1 (rat): Y15‑p, CDK1 (chicken): Y15‑p, CDK1 (fruit fly): Y15‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes MK1775 decrease

T210-p - PLK1 (human) ▲

Modsite: YDGERKktLCGtPNy SwissProt Entrez-Gene Orthologous residues PLK1 (human): T210‑p, PLK1 (mouse): T210‑p, PLK1 (rat): T210‑p, PLK1 (frog): T201‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human