Gordon SL, Bobrovskaya L, Dunkley PR, Dickson PW (2009) Differential regulation of human tyrosine hydroxylase isoforms 1 and 2 in situ: Isoform 2 is not phosphorylated at Ser35. Biochim Biophys Acta 1793, 1860-7 19833152

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S19-p - TH iso3 (human) ▲

Modsite: KGFRRAVsELDAKQA SwissProt Entrez-Gene Orthologous residues TH (human): S19‑p, TH iso2 (human): S19‑p, TH iso3 (human): S19‑p, TH iso4 (human): S19‑p, TH (mouse): S19‑p, TH (rat): S19‑p, TH (cow): S19‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  SH-SY5Y (neural crest) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes colforsin no change compared to control muscarine increase U0126 no change compared to control EGF no change compared to control

S31-p - TH iso3 (human) ▲

Modsite: KQAEAIMsPRFIGRR SwissProt Entrez-Gene Orthologous residues TH (human): S62‑p, TH iso2 (human): S58‑p, TH iso3 (human): S31‑p, TH iso4 (human): S35‑p, TH (mouse): S31‑p, TH (rat): S31‑p, TH (cow): S31‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  SH-SY5Y (neural crest) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes colforsin no change compared to control muscarine no change compared to control EGF increase U0126 decrease Downstream Regulation Effect of modification (function):  phosphorylation Comments:  stimulates phosphorylation of S40 in TH iso2

S40-p - TH iso3 (human) ▲

Modsite: RFIGRRQsLIEDARK SwissProt Entrez-Gene Orthologous residues TH (human): S71‑p, TH iso2 (human): S67‑p, TH iso3 (human): S40‑p, TH iso4 (human): S44‑p, TH (mouse): S40‑p, TH (rat): S40‑p, TH (cow): S40‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  SH-SY5Y (neural crest) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes colforsin increase muscarine increase EGF no change compared to control U0126 decrease

S19-p - TH iso4 (human) ▲

Modsite: KGFRRAVsELDAKQA SwissProt Entrez-Gene Orthologous residues TH (human): S19‑p, TH iso2 (human): S19‑p, TH iso3 (human): S19‑p, TH iso4 (human): S19‑p, TH (mouse): S19‑p, TH (rat): S19‑p, TH (cow): S19‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  SH-SY5Y (neural crest) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes colforsin no change compared to control muscarine increase U0126 no change compared to control EGF no change compared to control

S35-p - TH iso4 (human) ▲

Modsite: AIMVRGQsPRFIGRR SwissProt Entrez-Gene Orthologous residues TH (human): S62‑p, TH iso2 (human): S58‑p, TH iso3 (human): S31‑p, TH iso4 (human): S35‑p, TH (mouse): S31‑p, TH (rat): S31‑p, TH (cow): S31‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  SH-SY5Y (neural crest) Cellular systems studied:  cell lines Species studied:  human

S44-p - TH iso4 (human) ▲

Modsite: RFIGRRQsLIEDARK SwissProt Entrez-Gene Orthologous residues TH (human): S71‑p, TH iso2 (human): S67‑p, TH iso3 (human): S40‑p, TH iso4 (human): S44‑p, TH (mouse): S40‑p, TH (rat): S40‑p, TH (cow): S40‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  neuroblastoma Relevant cell lines - cell types - tissues:  SH-SY5Y (neural crest) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes colforsin increase muscarine increase EGF no change compared to control U0126 no change compared to control