Curated Information
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Home > Curated Information Page > PubMed Id: 19815529
Hoppe S, et al. (2009) AMP-activated protein kinase adapts rRNA synthesis to cellular energy supply. Proc Natl Acad Sci U S A 106, 17781-6 19815529
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S44-p - TIF1A (human)
Modsite: LENDFFNsPPRKTVR SwissProt Entrez-Gene
Orthologous residues
TIF1A (human): S44‑p, TIF1A (mouse): S42‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
acadesine no change compared to control

S199-p - TIF1A (human)
Modsite: IIARYVPstPWFLMP SwissProt Entrez-Gene
Orthologous residues
TIF1A (human): S199‑p, TIF1A (mouse): S197‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
acadesine increase

S635-p - TIF1A (human)
Modsite: DTHFRsPssSVGsPP SwissProt Entrez-Gene
Orthologous residues
TIF1A (human): S635‑p, TIF1A (mouse): S633‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AMPKA1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA1 (human) pharmacological activator of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
acadesine increase
glucose_starvation increase
rapamycin no change compared to control
Downstream Regulation
Effect of modification (function):  activity, inhibited
Effect of modification (process):  transcription, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
TAF1C (human) Disrupts co-immunoprecipitation
Comments:  inhibits transcription complex formation