Curated Information
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Home > Curated Information Page > PubMed Id: 30696771
Zhang Q, et al. (2019) Parathyroid hormone initiates dynamic NHERF1 phosphorylation cycling and conformational changes that regulate NPT2A-dependent phosphate transport. J Biol Chem 30696771
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S46-p - NHERF (human)
Modsite: IRLVEPGsPAEkAGL SwissProt Entrez-Gene
Orthologous residues
NHERF (human): S46‑p, NHERF (mouse): S46‑p, NHERF (rat): S46‑p, NHERF (rabbit): S46‑p, NHERF (dog): S46‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S162-p - NHERF (human)
Modsite: CtMKKGPsGyGFNLH SwissProt Entrez-Gene
Orthologous residues
NHERF (human): S162‑p, NHERF (mouse): N157‑p, NHERF (rat): N159‑p, NHERF (rabbit): N162‑p, NHERF (dog): N169‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S181-p - NHERF (human)
Modsite: KPGQFIRsVDPDsPA SwissProt Entrez-Gene
Orthologous residues
NHERF (human): S181‑p, NHERF (mouse): A176‑p, NHERF (rat): A178‑p, NHERF (rabbit): A181‑p, NHERF (dog): A188‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S269-p - NHERF (human)
Modsite: GEIQKENsREALAEA SwissProt Entrez-Gene
Orthologous residues
NHERF (human): S269‑p, NHERF (mouse): S264‑p, NHERF (rat): S266‑p, NHERF (rabbit): N268‑p, NHERF (dog): S277‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PTH(1-34) increase

S280-p - NHERF (human)
Modsite: LAEAALEsPRPALVR SwissProt Entrez-Gene
Orthologous residues
NHERF (human): S280‑p, NHERF (mouse): S275‑p, NHERF (rat): S277‑p, NHERF (rabbit): S279‑p, NHERF (dog): S288‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S290-p - NHERF (human)
Modsite: PALVRsAssDtsEEL SwissProt Entrez-Gene
Orthologous residues
NHERF (human): S290‑p, NHERF (mouse): S285‑p, NHERF (rat): S287‑p, NHERF (rabbit): S289‑p, NHERF (dog): S298‑p
Characterization
Methods used to characterize site in vivo [32P] ATP in vitro, [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MC3T3-E1 (preosteoblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP1CA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP1CA (human) co-immunoprecipitation, pharmacological inhibitor of upstream enzyme, transfection of constitutively active enzyme, transfection of wild-type enzyme
KINASE GRK6 (human) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PTH(1-34) increase
tautomycetin increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation, protein conformation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SLC34A1 (human) Induces co-immunoprecipitation
Comments:  regulates phosphate uptake by SLC34A1

S291-p - NHERF (human)
Modsite: ALVRsAssDtsEELN SwissProt Entrez-Gene
Orthologous residues
NHERF (human): S291‑p, NHERF (mouse): S286‑p, NHERF (rat): S288‑p, NHERF (rabbit): S290‑p, NHERF (dog): S299‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PTH(1-34) increase

T293-p - NHERF (human)
Modsite: VRsAssDtsEELNsQ SwissProt Entrez-Gene
Orthologous residues
NHERF (human): T293‑p, NHERF (mouse): T288‑p, NHERF (rat): T290‑p, NHERF (rabbit): T292‑p, NHERF (dog): T301‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PTH(1-34) increase

S299-p - NHERF (human)
Modsite: DtsEELNsQDsPPKQ SwissProt Entrez-Gene
Orthologous residues
NHERF (human): S299‑p, NHERF (mouse): S294‑p, NHERF (rat): A296‑p, NHERF (rabbit): S298‑p, NHERF (dog): S307‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S302-p - NHERF (human)
Modsite: EELNsQDsPPKQDST SwissProt Entrez-Gene
Orthologous residues
NHERF (human): S302‑p, NHERF (mouse): S297‑p, NHERF (rat): S299‑p, NHERF (rabbit): S301‑p, NHERF (dog): S310‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PTH(1-34) no change compared to control