Curated Information
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Home > Curated Information Page > PubMed Id: 11278940
Fan G, Shumay E, Malbon CC, Wang H (2001) c-Src tyrosine kinase binds the beta 2-adrenergic receptor via phospho-Tyr-350, phosphorylates G-protein-linked receptor kinase 2, and mediates agonist-induced receptor desensitization. J Biol Chem 276, 13240-7 11278940
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y419-p - Src (human)
Modsite: RLIEDNEytARQGAk SwissProt Entrez-Gene
Orthologous residues
Src (human): Y419‑p, Src iso2 (human): Y425‑p, Src (mouse): Y424‑p, Src iso2 (mouse): Y418‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  A431 (epithelial), CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster, human

Y132-p - ADRB2 (hamster)
Modsite: CVIAVDRyIAITSPF SwissProt Entrez-Gene
Orthologous residues
ADRB2 (human): Y132‑p, ADRB2 (mouse): Y132‑p, ADRB2 (rat): Y132‑p, ADRB2 (hamster): Y132‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  A431 (epithelial), CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase

Y141-p - ADRB2 (hamster)
Modsite: AITSPFKyQSLLTKN SwissProt Entrez-Gene
Orthologous residues
ADRB2 (human): Y141‑p, ADRB2 (mouse): Y141‑p, ADRB2 (rat): Y141‑p, ADRB2 (hamster): Y141‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  A431 (epithelial), CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase

Y350-p - ADRB2 (hamster)
Modsite: RRSSSKAyGNGySSN SwissProt Entrez-Gene
Orthologous residues
ADRB2 (human): Y350‑p, ADRB2 (mouse): Y350‑p, ADRB2 (rat): Y350‑p, ADRB2 (hamster): Y350‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  A431 (epithelial), CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation, receptor desensitization, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Src (mouse) Induces activity, induced

Y354-p - ADRB2 (hamster)
Modsite: SKAyGNGySSNSNGK SwissProt Entrez-Gene
Orthologous residues
ADRB2 (human): Y354‑p, ADRB2 (mouse): Y354‑p, ADRB2 (rat): Y354‑p, ADRB2 (hamster): Y354‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  A431 (epithelial), CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase

Y364-p - ADRB2 (hamster)
Modsite: NSNGKTDyMGEASGC SwissProt Entrez-Gene
Orthologous residues
ADRB2 (human): , ADRB2 (mouse): Y364‑p, ADRB2 (rat): Y364‑p, ADRB2 (hamster): Y364‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  A431 (epithelial), CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase