Curated Information
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Home > Curated Information Page > PubMed Id: 12370821
Leu TH, Maa MC (2002) Tyr-863 phosphorylation enhances focal adhesion kinase autophosphorylation at Tyr-397. Oncogene 21, 6992-7000 12370821
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y397-p - FAK (chicken)
Modsite: sVsETDDyAEIIDEE SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y397‑p, FAK iso2 (human): Y216‑p, FAK iso5 (human): Y397‑p, FAK (mouse): Y397‑p, FAK iso2 (mouse): Y428‑p, FAK iso4 (mouse): Y397‑p, FAK iso9 (mouse): , FAK (rat): Y397‑p, FAK (chicken): Y397‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  chicken
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE FAK (chicken) mutation in upstream enzyme recognition motif Expression of active Src is necessary. Both Y576/577 and Y863 can cooperatively regulate the phosphorylation of FAK at the same time.
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin increase

Y576-p - FAK (chicken)
Modsite: RyMEDSTyyKASKGK SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y576‑p, FAK iso2 (human): Y424‑p, FAK iso5 (human): Y576‑p, FAK (mouse): Y576‑p, FAK iso2 (mouse): Y607‑p, FAK iso4 (mouse): Y576‑p, FAK iso9 (mouse): , FAK (rat): Y576‑p, FAK (chicken): Y576‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  chicken
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, phosphorylation
Comments:  Regulates FAK autophosphorylation at Y397. Increases the intermolecular transphosphorylation activity of FAK (in vitro).

Y577-p - FAK (chicken)
Modsite: yMEDSTyyKASKGKL SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y577‑p, FAK iso2 (human): Y425‑p, FAK iso5 (human): Y577‑p, FAK (mouse): Y577‑p, FAK iso2 (mouse): Y608‑p, FAK iso4 (mouse): Y577‑p, FAK iso9 (mouse): , FAK (rat): Y577‑p, FAK (chicken): Y577‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  chicken
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, phosphorylation
Comments:  Regulates FAK autophosphorylation at Y397. Increases the intermolecular transphosphorylation activity of FAK (in vitro).

Y863-p - FAK (chicken)
Modsite: PAGNQHIyQPVGKPD SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y861‑p, FAK iso2 (human): Y688‑p, FAK iso5 (human): Y861‑p, FAK (mouse): Y861‑p, FAK iso2 (mouse): Y892‑p, FAK iso4 (mouse): Y861‑p, FAK iso9 (mouse): Y169‑p, FAK (rat): Y861‑p, FAK (chicken): Y863‑p, FAK iso5 (chicken): Y169‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'stem, embryonic'
Cellular systems studied:  primary cells
Species studied:  chicken
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, phosphorylation
Comments:  Regulates FAK autophosphorylation at Y397. It is crucial for FAK cis-phosphorylation (in vitro).