Curated Information
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Home > Curated Information Page > PubMed Id: 10025949
Chen ZP, et al. (1999) AMP-activated protein kinase phosphorylation of endothelial NO synthase. FEBS Lett 443, 285-9 10025949
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T494-p - eNOS (rat)
Modsite: TGITRKKtFKEVANA SwissProt Entrez-Gene
Orthologous residues
eNOS (human): T495‑p, eNOS (mouse): T494‑p, eNOS (rat): T494‑p, eNOS (rabbit): T501‑p, eNOS (pig): T497‑p, eNOS (cow): T497‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AMPKA1 (rat)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA1 (rat) co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  enzymatic activity, inhibited
Comments:  Phosphorylated In the absence of Ca2+ inhibiting eNOS, but not during ischaemia.

S1176-p - eNOS (rat)
Modsite: TSRIRtQsFsLQERQ SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S1177‑p, eNOS (mouse): S1176‑p, eNOS (rat): S1176‑p, eNOS (rabbit): S1183‑p, eNOS (pig): S1179‑p, eNOS (cow): S1179‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AMPKA1 (rat)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA1 (rat) co-immunoprecipitation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ischemia increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Comments:  Activated in vitro and during ischaemia in the presence of Ca2+ calmodulin.