Liu X, et al. (2019) Glycolipid iGb3 feedback amplifies innate immune responses via CD1d reverse signaling. Cell Res 29, 42-53 30514903

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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Y332-p - CD1D (mouse) ▲

Modsite: IWRRRSAyQDIR___ SwissProt Entrez-Gene Orthologous residues CD1D (human): Y331‑p, CD1D (mouse): Y332‑p, CD1D (rat): Y332‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), macrophage, macrophage-bone marrow Cellular systems studied:  cell lines, primary cells, primary cultured cells Species studied:  human, mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes LPS increase Increase after 15 minutes of incubation CIAP LPS inhibit treatment-induced increase PP1 LPS inhibit treatment-induced increase LPS A3GALT2 (mouse) augment treatment-induced increase A3GALT2 (mouse) no change compared to control poly(I-C) increase poly(I-C) A3GALT2 (mouse) augment treatment-induced increase CpG increase CpG A3GALT2 (mouse) augment treatment-induced increase LPS HEXB (mouse), A3GALT2 (mouse) augment treatment-induced increase A3GAL2 + HEXB siRNA inhibits treatment Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Pyk2 (mouse) Induces phosphorylation co-immunoprecipitation, microscopy-colocalization

Y188-p - IKKB (mouse) ▲

Modsite: sFVGTLQyLAPELLE SwissProt Entrez-Gene Orthologous residues IKKB (human): Y188‑p, IKKB (mouse): Y188‑p, IKKB (rat): Y188‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), macrophage Cellular systems studied:  cell lines, primary cells Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE Pyk2 (mouse) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Pyk2 (mouse) siRNA inhibition of enzyme, phospho-antibody, transfection of inactive enzyme, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes LPS increase LPS CD1D (mouse) augment treatment-induced increase CD1D KO inhibits poly(I-C) increase poly(I-C) CD1D (mouse) augment treatment-induced increase CD1D KO inhibits CpG increase CpG CD1D (mouse) augment treatment-induced increase CD1D KO inhibits LPS A3GALT2 (mouse) augment treatment-induced increase poly(I-C) A3GALT2 (mouse) augment treatment-induced increase CpG A3GALT2 (mouse) augment treatment-induced increase siRNA LPS inhibit treatment-induced increase PYK2 siRNA Downstream Regulation Effect of modification (function):  phosphorylation Effect of modification (process):  signaling pathway regulation Comments:  Y188/199 of IKKß and Y179 TBK1 phosphorylation induce activation of TLR signaling

Y199-p - IKKB (mouse) ▲

Modsite: ELLEQQKyTVTVDYW SwissProt Entrez-Gene Orthologous residues IKKB (human): Y199‑p, IKKB (mouse): Y199‑p, IKKB (rat): Y199‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), macrophage Cellular systems studied:  cell lines, primary cells Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE Pyk2 (mouse) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Pyk2 (mouse) siRNA inhibition of enzyme, phospho-antibody, transfection of inactive enzyme, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes LPS increase LPS CD1D (mouse) augment treatment-induced increase CD1D KO inhibits poly(I-C) increase poly(I-C) CD1D (mouse) augment treatment-induced increase CD1D KO inhibits CpG increase CpG CD1D (mouse) augment treatment-induced increase CD1D KO inhibits LPS A3GALT2 (mouse) augment treatment-induced increase poly(I-C) A3GALT2 (mouse) augment treatment-induced increase CpG A3GALT2 (mouse) augment treatment-induced increase siRNA LPS inhibit treatment-induced increase PYK2 siRNA Downstream Regulation Effect of modification (function):  phosphorylation Effect of modification (process):  signaling pathway regulation Comments:  Y188/199 of IKKß and Y179 TBK1 phosphorylation induce activation of TLR signaling

Y179-p - TBK1 (mouse) ▲

Modsite: sLYGTEEyLHPDMYE SwissProt Entrez-Gene Orthologous residues TBK1 (human): Y179‑p, TBK1 (mouse): Y179‑p, TBK1 (rat): Y179‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), macrophage Cellular systems studied:  cell lines, primary cells Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE Pyk2 (mouse) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Pyk2 (mouse) siRNA inhibition of enzyme, phospho-antibody, transfection of inactive enzyme, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes LPS increase LPS CD1D (mouse) augment treatment-induced increase CD1D KO inhibits poly(I-C) increase poly(I-C) CD1D (mouse) augment treatment-induced increase CD1D KO inhibits CpG increase CpG CD1D (mouse) augment treatment-induced increase CD1D KO inhibits LPS A3GALT2 (mouse) augment treatment-induced increase poly(I-C) A3GALT2 (mouse) augment treatment-induced increase CpG A3GALT2 (mouse) augment treatment-induced increase siRNA LPS inhibit treatment-induced increase PYK2 siRNA Downstream Regulation Effect of modification (function):  phosphorylation Effect of modification (process):  signaling pathway regulation Comments:  Y188/199 of IKKß and Y179 TBK1 phosphorylation induce activation of TLR signaling