Zheng S, et al. (2018) Regulation of erythrocyte Na/K/2Cl cotransport by an oxygen-switched kinase cascade. J Biol Chem 30563844

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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T205-p - NKCC1 (mouse) ▲

Modsite: TNtYYLRtFGHNtMD SwissProt Entrez-Gene Orthologous residues NKCC1 (human): T212‑p, NKCC1 iso3 (human): T212‑p, NKCC1 (mouse): T205‑p, NKCC1 (rat): T203‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  erythrocyte-blood Cellular systems studied:  primary cultured cells Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes hypoxia/reoxygenation increase oxygenation deoxygenation no change compared to control

T210-p - NKCC1 (mouse) ▲

Modsite: LRtFGHNtMDAVPRI SwissProt Entrez-Gene Orthologous residues NKCC1 (human): T217‑p, NKCC1 iso3 (human): T217‑p, NKCC1 (mouse): T210‑p, NKCC1 (rat): T208‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  erythrocyte-blood Cellular systems studied:  primary cultured cells Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes hypoxia/reoxygenation increase oxygenation deoxygenation no change compared to control

T185-p - OSR1 (mouse) ▲

Modsite: TRNKVRKtFVGtPCW SwissProt Entrez-Gene Orthologous residues OSR1 (human): T185‑p, OSR1 (mouse): T185‑p, OSR1 (rat): T185‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  erythrocyte-blood Cellular systems studied:  primary cultured cells Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes deoxygenation increase WNK1 (mouse) increase

S325-p - OSR1 (mouse) ▲

Modsite: VRRVPGssGRLHKtE SwissProt Entrez-Gene Orthologous residues OSR1 (human): S325‑p, OSR1 (mouse): S325‑p, OSR1 (rat): S325‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  erythrocyte-blood Cellular systems studied:  primary cultured cells Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes deoxygenation increase WNK1 (mouse) increase