Curated Information
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Home > Curated Information Page > PubMed Id: 9880482
Ahn S, et al. (1999) Src-mediated tyrosine phosphorylation of dynamin is required for beta2-adrenergic receptor internalization and mitogen-activated protein kinase signaling. J Biol Chem 274, 1185-8 9880482
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y231-p - DYN1 (rat)
Modsite: LLPLRRGyIGVVNRS SwissProt Entrez-Gene
Orthologous residues
DYN1 (human): Y231‑p, DYN1 iso2 (human): Y231‑p, DYN1 iso3 (human): Y231‑p, DYN1 (mouse): Y231‑p, DYN1 iso3 (mouse): Y231‑p, DYN1 iso4 (mouse): Y231‑p, DYN1 iso6 (mouse): Y231‑p, DYN1 (rat): Y231‑p, DYN1 iso2 (rat): Y231‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (rat) transfection of inactive enzyme, transfection of constitutively active enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase
Downstream Regulation
Effect of modification (function):  activity, inhibited, molecular association, regulation, receptor internalization, altered
Comments:  Y231/597F mutant Inhibits B2-AR internalization, and inhibited the isoproterenol mediated activation of ERK2.

Y597-p - DYN1 (rat)
Modsite: NTEQRNVykDYRQLE SwissProt Entrez-Gene
Orthologous residues
DYN1 (human): Y597‑p, DYN1 iso2 (human): Y597‑p, DYN1 iso3 (human): Y597‑p, DYN1 (mouse): Y597‑p, DYN1 iso3 (mouse): Y597‑p, DYN1 iso4 (mouse): Y597‑p, DYN1 iso6 (mouse): Y593‑p, DYN1 (rat): Y597‑p, DYN1 iso2 (rat): Y597‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (rat) transfection of inactive enzyme, transfection of constitutively active enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
isoproterenol increase
Downstream Regulation
Effect of modification (function):  activity, inhibited, molecular association, regulation, receptor internalization, altered
Comments:  Y231/597F mutant Inhibits B2-AR internalization, and inhibited the isoproterenol mediated activation of ERK2.