Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 30377223
Artamonov MV, et al. (2018) RSK2 contributes to myogenic vasoconstriction of resistance arteries by activating smooth muscle myosin and the Na/H exchanger. Sci Signal 11 30377223
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S19-p - MRLC3 (human)
Modsite: KRPQRAtsNVFAMFD SwissProt Entrez-Gene
Orthologous residues
MRLC3 (human): S19‑p, MRLC3 (mouse): S20‑p, MRLC3 (rat): S20‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, smooth', small mesenteric arteries
Cellular systems studied:  tissue
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE RSK2 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE RSK2 (human) phospho-antibody, genetic knockout/knockin of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
blood pressure increase phosphorylation increased significantly within 30s at 80 mmHg
endothelin increase Increase Phosphorylation on 30 seconds to 90 seconds followed by decrease after 5 mins
angiotensin_2 increase Increase Phosphorylation on 30 seconds to 90 seconds followed by decrease after 5 mins
U46619 increase
phenylephrine increase Increase Phosphorylation on 30 seconds to 90 seconds followed by decrease after 5 mins

T852-p - MYPT1 (mouse)
Modsite: PREKRRstGVSFWtQ SwissProt Entrez-Gene
Orthologous residues
MYPT1 (human): T853‑p, MYPT1 iso2 (human): T853‑p, MYPT1 iso3 (human): T797‑p, MYPT1 iso4 (human): T794‑p, MYPT1 (mouse): T852‑p, MYPT1 (rat): T855‑p, MYPT1 (pig): , MYPT1 (chicken): T850‑p, MYPT1 (cow): T851‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, smooth', small mesenteric arteries
Cellular systems studied:  tissue
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ROCK2 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
endothelin increase
angiotensin_2 increase Increase Phosphorylation on 30 seconds to 90 seconds followed by decrease after after 90 seconds (5 mins)
U46619 increase
phenylephrine increase Increase Phosphorylation on 30 seconds slowly decrease after 90 seconds to 5 mins
blood pressure increase Increase Phosphorylation on 30 seconds to 90 seconds followed by decrease slowly there after (5 mins)
RSK2 (human) no change compared to control RSK2 knockout has no effect
ROCK2 (human) increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, inhibited