Mo SJ, et al. (2019) PKA-dependent phosphorylation of IP3K-A at Ser119 regulates a binding affinity with EB3. Biochem Biophys Res Commun 508, 52-59 30466786

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S119-p - ITPKA (rat) ▲

Modsite: LQQPRRLsTSSLSST SwissProt Entrez-Gene Orthologous residues ITPKA (human): S121‑p, ITPKA (mouse): S119‑p, ITPKA (rat): S119‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  'neuron, hippocampal', HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human, rat Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKACA (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKACA (human) pharmacological activator of upstream enzyme, pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes NKH_477 increase H-89 decrease long-term_potentiation increase increase followed by decrease Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cytoskeletal reorganization, neural plasticity Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays EB3 (human) Disrupts co-immunoprecipitation, in vitro, pull-down assay, microscopy-colocalization