Carmona-Rosas G, et al. (2019) Distinct phosphorylation sites/clusters in the carboxyl terminus regulate α-adrenergic receptor subcellular localization and signaling. Cell Signal 53, 374-389 30419287

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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T442-p - ADRA1D (human) ▲

Modsite: GHHWRAStSGLRQDC SwissProt Entrez-Gene Orthologous residues ADRA1D (human): T442‑p, ADRA1D (mouse): L430‑p, ADRA1D (rat): T434‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase norepinephrine increase

T477-p - ADRA1D (human) ▲

Modsite: PDPEPPGtPEMQAPV SwissProt Entrez-Gene Orthologous residues ADRA1D (human): T477‑p, ADRA1D (mouse): L464‑p, ADRA1D (rat): T467‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase norepinephrine no change compared to control

S486-p - ADRA1D (human) ▲

Modsite: EMQAPVAsRRKPPsA SwissProt Entrez-Gene Orthologous residues ADRA1D (human): S486‑p, ADRA1D (mouse): F473‑p, ADRA1D (rat): S476‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase norepinephrine no change compared to control

S492-p - ADRA1D (human) ▲

Modsite: AsRRKPPsAFREWRL SwissProt Entrez-Gene Orthologous residues ADRA1D (human): S492‑p, ADRA1D (mouse): K479‑p, ADRA1D (rat): S482‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase norepinephrine no change compared to control

T507-p - ADRA1D (human) ▲

Modsite: LGPFRRPtTQLRAKV SwissProt Entrez-Gene Orthologous residues ADRA1D (human): T507‑p, ADRA1D (mouse): T497‑p, ADRA1D (rat): T497‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase norepinephrine increase Downstream Regulation Effect of modification (function):  intracellular localization, molecular association, regulation, receptor internalization, induced Effect of modification (process):  signaling pathway regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays ARRB1 (human) Induces microscopy-colocalization Comments:  triggers the calcium signaling pathway

S515-p - ADRA1D (human) ▲

Modsite: TQLRAKVssLsHKIR SwissProt Entrez-Gene Orthologous residues ADRA1D (human): S515‑p, ADRA1D (mouse): S505‑p, ADRA1D (rat): S505‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase norepinephrine increase Downstream Regulation Effect of modification (function):  intracellular localization, molecular association, regulation, receptor internalization, induced Effect of modification (process):  signaling pathway regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays ARRB1 (human) Induces microscopy-colocalization Comments:  triggers the calcium signaling pathway

S516-p - ADRA1D (human) ▲

Modsite: QLRAKVssLsHKIRA SwissProt Entrez-Gene Orthologous residues ADRA1D (human): S516‑p, ADRA1D (mouse): S506‑p, ADRA1D (rat): S506‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase norepinephrine increase Downstream Regulation Effect of modification (function):  intracellular localization, molecular association, regulation, receptor internalization, induced Effect of modification (process):  signaling pathway regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays ARRB1 (human) Induces microscopy-colocalization Comments:  triggers the calcium signaling pathway

S518-p - ADRA1D (human) ▲

Modsite: RAKVssLsHKIRAGG SwissProt Entrez-Gene Orthologous residues ADRA1D (human): S518‑p, ADRA1D (mouse): S508‑p, ADRA1D (rat): S508‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase norepinephrine increase Downstream Regulation Effect of modification (function):  intracellular localization, molecular association, regulation, receptor internalization, induced Effect of modification (process):  signaling pathway regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays ARRB1 (human) Induces microscopy-colocalization Comments:  triggers the calcium signaling pathway

S543-p - ADRA1D (human) ▲

Modsite: RSEVEAVsLGVPHEV SwissProt Entrez-Gene Orthologous residues ADRA1D (human): S543‑p, ADRA1D (mouse): S533‑p, ADRA1D (rat): S532‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase norepinephrine no change compared to control