Curated Information
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Home > Curated Information Page > PubMed Id: 10669737
Michael LF, et al. (2000) The phosphorylation status of a cyclic AMP-responsive activator is modulated via a chromatin-dependent mechanism. Mol Cell Biol 20, 1596-603 10669737
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S63-p - ATF-1 (mouse)
Modsite: GILARRPsYRKILKD SwissProt Entrez-Gene
Orthologous residues
ATF‑1 (human): S63‑p, ATF‑1 (mouse): S63‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  D5 (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
colforsin, trichostatin_A, butyrate increase HDAC inhibitors with costimulation with cAMP agonist, prolongs CREB S133 and ATF-1 S63 phosphorylation. Forskolin withdrwal resulted in a half-life of CREB phosphorylation of 30 min. Phosphatase activity was not shown to to be affected by HDAC inhibitors.

S133-p - CREB (mouse)
Modsite: EILsRRPsyRkILND SwissProt Entrez-Gene
Orthologous residues
CREB (human): S133‑p, CREB iso2 (human): S119‑p, CREB (mouse): S133‑p, CREB (rat): S133‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  D5 (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
colforsin, trichostatin_A, butyrate increase HDAC inhibitors with costimulation with cAMP agonist, prolongs CREB S133 and ATF-1 S63 phosphorylation. Forskolin withdrwal resulted in a half-life of CREB phosphorylation of 30 min. Phosphatase activity was not shown to to be affected by HDAC inhibitors.
Downstream Regulation
Effect of modification (function):  phosphorylation
Effect of modification (process):  transcription, induced
Comments:  Chomatin localization was imprortant for regulation of CREB by PKA.

S84-p - CREM iso2 (mouse)
Modsite: EILSRRPsYRKILNE SwissProt Entrez-Gene
Orthologous residues
CREM (human): S120‑p, CREM iso2 (human): S87‑p, CREM iso6 (human): S136‑p, CREM iso8 (human): , CREM (mouse): S133‑p, CREM iso2 (mouse): S84‑p, CREM iso3 (mouse): S84‑p