Curated Information
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Home > Curated Information Page > PubMed Id: 7731718
Pelicci G, et al. (1995) The motogenic and mitogenic responses to HGF are amplified by the Shc adaptor protein. Oncogene 10, 1631-8 7731718
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y1349-p - Met (human)
Modsite: stFIGEHyVHVNAty SwissProt Entrez-Gene
Orthologous residues
Met (human): Y1349‑p, Met iso2 (human): Y1367‑p, Met (mouse): Y1347‑p, Met (rat): Y1350‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  lung cancer
Relevant cell lines - cell types - tissues:  A549 (pulmonary), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SHC1 (human) SH2 Induces co-immunoprecipitation
Comments:  association in vitro and in vivo

Y1356-p - Met (human)
Modsite: yVHVNAtyVNVKCVA SwissProt Entrez-Gene
Orthologous residues
Met (human): Y1356‑p, Met iso2 (human): Y1374‑p, Met (mouse): Y1354‑p, Met (rat): Y1357‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  lung cancer
Relevant cell lines - cell types - tissues:  A549 (pulmonary), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SHC1 (human) SH2 Induces co-immunoprecipitation
Comments:  association in vitro and in vivo

Y427-p - SHC1 (human)
Modsite: ELFDDPsyVNVQNLD SwissProt Entrez-Gene
Orthologous residues
SHC1 (human): Y427‑p, SHC1 iso2 (human): Y317‑p, SHC1 iso3 (human): Y272‑p, SHC1 iso6 (human): Y428‑p, SHC1 iso7 (human): Y318‑p, SHC1 (mouse): Y423‑p, SHC1 iso2 (mouse): Y313‑p, SHC1 iso3 (mouse): Y268‑p, SHC1 (rat): Y423‑p, SHC1 iso2 (rat): Y313‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  lung cancer
Relevant cell lines - cell types - tissues:  A549 (pulmonary), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Met (human) mutation in upstream enzyme recognition motif, co-immunoprecipitation, transfection of inactive enzyme
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cell growth, altered, cell motility, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
GRB2 (human) Induces in vitro