Curated Information
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Home > Curated Information Page > PubMed Id: 9630228
Zhu J, et al. (1998) Intramolecular masking of nuclear import signal on NF-AT4 by casein kinase I and MEKK1. Cell 93, 851-61 9630228
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T204-p - NFAT4 (human)
Modsite: NEAAARFtLGsPLts SwissProt Entrez-Gene
Orthologous residues
NFAT4 (human): T204‑p, NFAT4 iso2 (human): T204‑p, NFAT4 (mouse): T204‑p, NFAT4 iso3 (mouse): T204‑p, NFAT4 (rat): T205‑p
Characterization
Methods used to characterize site in vivo western blotting
Relevant cell lines - cell types - tissues:  BHK (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1A (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1A (human) transfection of dominant-negative enzyme, pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme, activation of upstream enzyme, microscopy-colocalization, mutation in upstream enzyme recognition motif
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  cytoplasmic localization (inhibition of nuclear localization)

S207-p - NFAT4 (human)
Modsite: AARFtLGsPLtsPGG SwissProt Entrez-Gene
Orthologous residues
NFAT4 (human): S207‑p, NFAT4 iso2 (human): S207‑p, NFAT4 (mouse): S207‑p, NFAT4 iso3 (mouse): S207‑p, NFAT4 (rat): S208‑p
Characterization
Methods used to characterize site in vivo western blotting
Relevant cell lines - cell types - tissues:  BHK (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1A (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1A (human) transfection of dominant-negative enzyme, pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme, activation of upstream enzyme, microscopy-colocalization, mutation in upstream enzyme recognition motif
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  cytoplasmic localization (inhibition of nuclear localization)

T210-p - NFAT4 (human)
Modsite: FtLGsPLtsPGGsPG SwissProt Entrez-Gene
Orthologous residues
NFAT4 (human): T210‑p, NFAT4 iso2 (human): T210‑p, NFAT4 (mouse): T210‑p, NFAT4 iso3 (mouse): T210‑p, NFAT4 (rat): T211‑p
Characterization
Methods used to characterize site in vivo western blotting
Relevant cell lines - cell types - tissues:  BHK (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1A (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1A (human) transfection of dominant-negative enzyme, pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme, activation of upstream enzyme, microscopy-colocalization, mutation in upstream enzyme recognition motif
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  cytoplasmic localization (inhibition of nuclear localization)

S211-p - NFAT4 (human)
Modsite: tLGsPLtsPGGsPGG SwissProt Entrez-Gene
Orthologous residues
NFAT4 (human): S211‑p, NFAT4 iso2 (human): S211‑p, NFAT4 (mouse): S211‑p, NFAT4 iso3 (mouse): S211‑p, NFAT4 (rat): S212‑p
Characterization
Methods used to characterize site in vivo western blotting
Relevant cell lines - cell types - tissues:  BHK (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1A (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1A (human) transfection of dominant-negative enzyme, pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme, activation of upstream enzyme, microscopy-colocalization, mutation in upstream enzyme recognition motif
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  cytoplasmic localization (inhibition of nuclear localization)

S215-p - NFAT4 (human)
Modsite: PLtsPGGsPGGCPGE SwissProt Entrez-Gene
Orthologous residues
NFAT4 (human): S215‑p, NFAT4 iso2 (human): S215‑p, NFAT4 (mouse): S215‑p, NFAT4 iso3 (mouse): S215‑p, NFAT4 (rat): S216‑p
Characterization
Methods used to characterize site in vivo western blotting
Relevant cell lines - cell types - tissues:  BHK (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CK1A (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK1A (human) transfection of dominant-negative enzyme, pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme, activation of upstream enzyme, microscopy-colocalization, mutation in upstream enzyme recognition motif
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  cytoplasmic localization (inhibition of nuclear localization)