Curated Information
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Home > Curated Information Page > PubMed Id: 10373465
Lam MH, et al. (1999) Phosphorylation at the cyclin-dependent kinases site (Thr85) of parathyroid hormone-related protein negatively regulates its nuclear localization. J Biol Chem 274, 18559-66 10373465
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T121-p - PTHrP (human)
Modsite: YKEQPLKtPGKKKKG SwissProt Entrez-Gene
Orthologous residues
PTHrP (human): T121‑p, PTHrP (mouse): T121‑p, PTHrP (rat): T121‑p
Characterization
Methods used to characterize site in vivo 2D analysis, flow cytometry, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK2 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK2 (human) modification site within consensus motif, phosphopeptide analysis, 2D analysis
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  T121E mutation was localized in the cytoplasm, while a T121A mutation showed nuclear localization.