Tyr1186
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Phosphorylation Site Page:
Tyr1186 - InsR (rat)

Site Information
FGMTRDIyETDYYRK    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: InsR (human): Y1185, InsR iso2 (human): Y1173, InsR (mouse): Y1175
Blast this site against: NCBI  SwissProt  PDB 

In vivo Characterization
Methods used to characterize site in vivo: [32P] bio-synthetic labeling (17), mass spectrometry (6, 7, 8, 9, 10, 11), phospho-antibody (5, 12, 13, 14, 15), phosphopeptide mapping (17), western blotting (5, 12, 13, 14, 15)
Disease tissue studied: liver cancer (17)
Relevant cell line - cell type - tissue: adipocyte-adipose tissue (14), brain (6, 7, 10), Fao (hepatic) (17), liver (5, 12, 13), PC-12 (chromaffin) (8, 9, 11), vascular smooth muscle cell ('muscle, smooth') (15)

Controlled by
Kinases, in vitro: InsR (rat) (16, 17)
Treatments: GRGDSP (13), hemorrhage (12), insulin (5, 12, 13, 14), irbesartan (5), lysophosphatidylcholine (15), PP2 (13), trauma (12), wortmannin (14)



References

1

Jun HJ, et al. (2012) The oncogenic lung cancer fusion kinase CD74-ROS activates a novel invasiveness pathway through E-Syt1 phosphorylation. Cancer Res 72, 3764-74
22659450   Curated Info

2

(2009) CST Curation Set: 8467; Year: 2009; SILAC: N; Biosample/Treatment: ST-31-BL(tissue)/untreated; Disease: ischemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

(2009) CST Curation Set: 8461; Year: 2009; SILAC: N; Biosample/Treatment: ST-23-BL(tissue)/untreated; Disease: ischemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

(2009) CST Curation Set: 8435; Year: 2009; SILAC: N; Biosample/Treatment: ST-6-BL(tissue)/untreated; Disease: ischemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Muñoz MC, et al. (2009) TANK-binding kinase 1 mediates phosphorylation of insulin receptor at serine residue 994: a potential link between inflammation and insulin resistance. J Endocrinol 201, 185-97
19251743   Curated Info

6

(2009) CST Curation Set: 5862; Year: 2009; SILAC: N; Biosample/Treatment: brain(tissue)/sham; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

(2009) CST Curation Set: 5864; Year: 2009; SILAC: N; Biosample/Treatment: brain(tissue)/seizure; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

(2008) CST Curation Set: 5264; Year: 2008; SILAC: N; Biosample/Treatment: PC-12(cell line)/untreated; Disease: pheochromocytoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

(2008) CST Curation Set: 5266; Year: 2008; SILAC: N; Biosample/Treatment: PC-12(cell line)/PACAP; Disease: pheochromocytoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

(2008) CST Curation Set: 5222; Year: 2008; SILAC: N; Biosample/Treatment: brain(tissue)/ischemia; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

11

(2008) CST Curation Set: 4012; Year: 2008; SILAC: N; Biosample/Treatment: PC-12(cell line)/untreated; Disease: pheochromocytoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

12

Ma Y, et al. (2004) Mechanisms of hemorrhage-induced hepatic insulin resistance: role of tumor necrosis factor-alpha. Endocrinology 145, 5168-76
15297437   Curated Info

13

Schliess F, et al. (2004) Involvement of integrins and Src in insulin signaling toward autophagic proteolysis in rat liver. J Biol Chem 279, 21294-301
14985360   Curated Info

14

Hers I, et al. (2002) Reciprocal feedback regulation of insulin receptor and insulin receptor substrate tyrosine phosphorylation by phosphoinositide 3-kinase in primary adipocytes. Biochem J 368, 875-84
12220227   Curated Info

15

Motley ED, et al. (2002) Lysophosphatidylcholine inhibits insulin-induced Akt activation through protein kinase C-alpha in vascular smooth muscle cells. Hypertension 39, 508-12
11882599   Curated Info

16

Kruszynska YT, Halban PA, Kahn CR, White MF (1990) Activation of liver and muscle insulin receptor tyrosine kinase activity during in vivo insulin administration in rats. Diabetologia 33, 77-83
2158467   Curated Info

17

White MF, Shoelson SE, Keutmann H, Kahn CR (1988) A cascade of tyrosine autophosphorylation in the beta-subunit activates the phosphotransferase of the insulin receptor. J Biol Chem 263, 2969-80
2449432   Curated Info

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