Thr1530

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Phosphorylation Site Page:

Thr1530 - ITGB4 (human)

Site Information

LTAGVPDtPTRLVFS SwissProt Entrez-Gene

Predicted information: Scansite

Orthologous residues: ITGB4 iso2 (human): T1460, ITGB4 (rat): T1514, ITGB4 iso2 (mouse): T1461, ITGB4 iso3 (human): T1513, ITGB4 (mouse): T1526, ITGB4 iso3 (mouse): T1514

Blast this site against: NCBI SwissProt PDB

In vivo Characterization

Methods used to characterize site in vivo:	mass spectrometry (1, 2, 3, 4, 5, 6, 7, 8, 9)
Disease tissue studied:	gastric cancer (1, 2, 3, 4), gastric carcinoma (1, 2, 3, 4)
Relevant cell line - cell type - tissue:	HeLa (cervical) (7), HeLa S3 (cervical) (6), HT-29 (intestinal) (8, 9), MKN-45 (gastric) (1, 2, 3, 4), MV4-11 (myeloid) (5)

	References

1	(2011) CST Curation Set: 11301; Year: 2011; Biosample: MKN-45; Disease: gastric carcinoma; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-RXX(s/t)(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS/T) Immunoaffinity Beads Cat#: 1978 Curated Info
2	(2011) CST Curation Set: 11325; Year: 2011; Biosample: MKN-45; Disease: gastric carcinoma; Treatment: normal; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-RXX(s/t)(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS/T) Immunoaffinity Beads Cat#: 1978 Curated Info
3	(2011) CST Curation Set: 11327; Year: 2011; Biosample: MKN-45; Disease: gastric carcinoma; Treatment: normal; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-RXX(s/t)(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS/T) Immunoaffinity Beads Cat#: 1978 Curated Info
4	(2011) CST Curation Set: 11330; Year: 2011; Biosample: MKN-45; Disease: gastric carcinoma; Treatment: normal; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-RXX(s/t)(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS/T) Immunoaffinity Beads Cat#: 1978 Curated Info
5	Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64 19369195 Curated Info
6	Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48 18691976 Curated Info
7	Beausoleil SA, et al. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nat Biotechnol 24, 1285-92 16964243 Curated Info
8	(2005) CST Curation Set: 658; Year: 2005; Biosample: HT-29; Disease: colorectal carcinoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-PXtP(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-MAPK Substrates (PXTP) (46G11) Rabbit mAb Cat#: 4391, PTMScan(R) Phospho-MAPK Substrate Motif (PXpTP) Immunoaffinity Beads Cat#: 1983 Curated Info
9	(2005) CST Curation Set: 800; Year: 2005; Biosample: HT-29; Disease: colorectal carcinoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-PXtP(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-MAPK Substrates (PXTP) (46G11) Rabbit mAb Cat#: 4391, PTMScan(R) Phospho-MAPK Substrate Motif (PXpTP) Immunoaffinity Beads Cat#: 1983 Curated Info

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