Ser483
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Phosphorylation Site Page:
Ser483 - B-Raf (mouse)

Site Information
KTLGRRDsSDDWEIP    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: B‑Raf (human): S446, B‑Raf (chicken): S486, B‑Raf (quail): S486, B‑Raf (rat): S483, B‑Raf iso3 (mouse):
Blast this site against: NCBI  SwissProt  PDB 

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (1, 2, 3, 4, 5, 7, 8, 9, 10, 12, 14, 15, 16, 17, 18, 20), phospho-antibody (6, 21), western blotting (6, 21)
Disease tissue studied: anthrax (7), melanoma skin cancer (16)
Relevant cell line - cell type - tissue: 'brain, forebrain' (17), 'fat, brown'-'fat, brown' (8), 32Dcl3 (myeloid) (12), 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ~aa 525-695 ETILLNS...IFEYCC)] (12), brain (1, 2, 3, 8, 10, 18), COS (fibroblast) (21), embryo (3), fibroblast-lung (14, 15), heart (8), HUVEC (endothelial) (6), kidney (8), liver (3, 8, 20), lung (8), macrophage-bone marrow (9), macrophage-bone marrow [MKP-1 (mouse), homozygous knockout] (9), MEF (fibroblast) (5), MEF (fibroblast) [p53 (mouse), homozygous knockout] (4), MEF (fibroblast) [TSC2 (mouse), homozygous knockout] (5), pancreas (8), PC-12 (chromaffin) (21), skin [mGluR1 (mouse), transgenic, TG mutant mice] (16), spleen (7, 8), testis (8)




References

1

Trinidad JC, et al. (2012) Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse. Mol Cell Proteomics 11, 215-29
22645316   Curated Info

2

Goswami T, et al. (2012) Comparative phosphoproteomic analysis of neonatal and adult murine brain. Proteomics 12, 2185-9
22807455   Curated Info

3

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

4

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

5

Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6
21659605   Curated Info

6

Xu K, et al. (2011) Blood vessel tubulogenesis requires Rasip1 regulation of GTPase signaling. Dev Cell 20, 526-39
21396893   Curated Info

7

Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927
21189417   Curated Info

8

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

9

Weintz G, et al. (2010) The phosphoproteome of toll-like receptor-activated macrophages. Mol Syst Biol 6, 371
20531401   Curated Info

10

Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9
20415495   Curated Info

11

(2010) CST Curation Set: 9035; Year: 2010; Biosample: mouse lung fibroblasts; Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-RXX(s/t)(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

12

Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39
19854140   Curated Info

13

(2009) CST Curation Set: 8045; Year: 2011; Biosample: mouse liver; Disease: -; Treatment: control; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-RXX(s/t)(phosphorylation)
Curated Info

14

(2009) CST Curation Set: 6376; Year: 2009; Biosample: fibroblast(lung); Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-RXX(s/t)(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

15

(2009) CST Curation Set: 6374; Year: 2009; Biosample: fibroblast(lung); Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-RXX(s/t)(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

16

Zanivan S, et al. (2008) Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-26
19367708   Curated Info

17

Collins MO, et al. (2008) Phosphoproteomic analysis of the mouse brain cytosol reveals a predominance of protein phosphorylation in regions of intrinsic sequence disorder. Mol Cell Proteomics 7, 1331-48
18388127   Curated Info

18

(2008) CST Curation Set: 3852; Year: 2008; Biosample: brain; Disease: -; Treatment: normal; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-RRX(s/t)(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) PKA Substrate Antibody Cat#: 9621
Curated Info

19

(2008) CST Curation Set: 3824; Year: 2008; Biosample: brain, embryonic; Disease: -; Treatment: normal; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-RXX(s/t)(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

20

Villén J, Beausoleil SA, Gerber SA, Gygi SP (2007) Large-scale phosphorylation analysis of mouse liver. Proc Natl Acad Sci U S A 104, 1488-93
17242355   Curated Info

21

Mason CS, et al. (1999) Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation. EMBO J 18, 2137-48
10205168   Curated Info

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