Lys328

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Acetylation Site Page:

Site Information

ALAPSTMkIKIIAPP SwissProt Entrez-Gene

Predicted information: Scansite

Orthologous residues: ACTC1 (human): K328, ACTC1 (rat): K328

Blast this site against: NCBI SwissProt PDB

In vivo Characterization

Methods used to characterize site in vivo:	mass spectrometry (1, 2, 3)
Relevant cell line - cell type - tissue:	colon (1), liver (1, 2), MEF (fibroblast) [HDAC6 (mouse), heterozygous knockout] (3)

Controlled by

Treatments:

gut microbiota (1)

	References

1	Simon GM, Cheng J, Gordon JI (2012) Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice. Proc Natl Acad Sci U S A 109, 11133-8 22733758 Curated Info
2	(2009) CST Curation Set: 7494; Year: 2009; Biosample: liver; Disease: -; Treatment: 0.1% serum; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-AcK Antibody Used to Purify Peptides prior to MS2: Acetylated-Lysine (Ac-K2-100) Rabbit mAb Cat#: 9814, PTMScan(R) Acetyl-Lys Motif (Ac-K) Immunoaffinity Beads Cat#: 1989 Curated Info
3	(2006) CST Curation Set: 1907; Year: 2006; Biosample: MEF[HDAC6(heterozygous knockout)]; Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-AcK Antibody Used to Purify Peptides prior to MS2: Acetylated-Lysine (Ac-K2-100) Rabbit mAb Cat#: 9814, PTMScan(R) Acetyl-Lys Motif (Ac-K) Immunoaffinity Beads Cat#: 1989 Curated Info

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