Ser135
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Phosphorylation Site Page:
Ser135 - BRAF (mouse)

Site Information
ASRNNPKsPQKPIVR    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: BRAF (rat): S134, BRAF (human): S151, BRAF (quail): S151, BRAF (chicken): S151, BRAF iso3 (mouse): S85
Blast this site against: NCBI  SwissProt  PDB 

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (1, 2, 10, 11, 12, 13)
Disease tissue studied: leukemia (2), acute myelogenous leukemia (2)
Relevant cell line - cell type - tissue: 'fat, brown'-'fat, brown' (10), 32Dcl3 (myeloid) (13), 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ~aa 525-695 ETILLNS...IFEYCC)] (13), blood (2), brain (12), kidney (10), lung (10), macrophage-bone marrow (11), macrophage-bone marrow [MKP-1 (mouse), homozygous knockout] (11), MEF (fibroblast) (1), MEF (fibroblast) [Raptor (mouse), knockdown] (1), MEF (fibroblast) [Rictor (mouse), knockdown] (1), pancreas (10)

Controlled by
Regulatory protein: Raptor (mouse) (1)
Treatments: LPS (11)



References

1

Robitaille AM, et al. (2013) Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis. Science 339, 1320-3
23429704   Curated Info

2

Trost M, et al. (2012) Posttranslational regulation of self-renewal capacity: insights from proteome and phosphoproteome analyses of stem cell leukemia. Blood 120, e17-27
22802335   Curated Info

3

(2011) CST Curation Set: 12732; Year: 2011; SILAC: N; Biosample/Treatment: brain(tissue)/untreated; Disease: brain cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser) CDKs Substrate Antibody Cat#: 2324, PTMScan(R) Phospho-CDK Substrate Motif (K/RS*PXK/R) Immunoaffinity Beads Cat#: 1981
Curated Info

4

(2011) CST Curation Set: 12733; Year: 2011; SILAC: N; Biosample/Treatment: brain(tissue)/untreated; Disease: brain cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser) CDKs Substrate Antibody Cat#: 2324, PTMScan(R) Phospho-CDK Substrate Motif (K/RS*PXK/R) Immunoaffinity Beads Cat#: 1981
Curated Info

5

(2011) CST Curation Set: 12734; Year: 2011; SILAC: N; Biosample/Treatment: brain(tissue)/untreated; Disease: brain cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser) CDKs Substrate Antibody Cat#: 2324, PTMScan(R) Phospho-CDK Substrate Motif (K/RS*PXK/R) Immunoaffinity Beads Cat#: 1981
Curated Info

6

(2011) CST Curation Set: 12736; Year: 2011; SILAC: N; Biosample/Treatment: brain(tissue)/untreated; Disease: brain cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-PXsP(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXS*P, S*PXK/R) Immunoaffinity Beads Cat#: 1982
Curated Info

7

(2011) CST Curation Set: 12737; Year: 2011; SILAC: N; Biosample/Treatment: brain(tissue)/untreated; Disease: brain cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-PXsP(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXS*P, S*PXK/R) Immunoaffinity Beads Cat#: 1982
Curated Info

8

(2011) CST Curation Set: 12738; Year: 2011; SILAC: N; Biosample/Treatment: brain(tissue)/untreated; Disease: brain cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-PXsP(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXS*P, S*PXK/R) Immunoaffinity Beads Cat#: 1982
Curated Info

9

(2011) CST Curation Set: 12739; Year: 2011; SILAC: N; Biosample/Treatment: brain(tissue)/untreated; Disease: brain cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-PXsP(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXS*P, S*PXK/R) Immunoaffinity Beads Cat#: 1982
Curated Info

10

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

11

Weintz G, et al. (2010) The phosphoproteome of toll-like receptor-activated macrophages. Mol Syst Biol 6, 371
20531401   Curated Info

12

Wiƛniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9
20415495   Curated Info

13

Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39
19854140   Curated Info

14

(2008) CST Curation Set: 4786; Year: 2008; SILAC: N; Biosample/Treatment: heart(tissue)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser) CDKs Substrate Antibody Cat#: 2324, PTMScan(R) Phospho-CDK Substrate Motif (K/RS*PXK/R) Immunoaffinity Beads Cat#: 1981
Curated Info

15

(2008) CST Curation Set: 4787; Year: 2008; SILAC: N; Biosample/Treatment: liver(tissue)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser) CDKs Substrate Antibody Cat#: 2324, PTMScan(R) Phospho-CDK Substrate Motif (K/RS*PXK/R) Immunoaffinity Beads Cat#: 1981
Curated Info

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