Thr1200
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Phosphorylation Site Page:
Thr1200 - SF3B3 (human)

Site Information
VSEELDRtPPEVSKK    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: SF3B3 (mouse): T1200
Blast this site against: NCBI  SwissProt  PDB 

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (2, 3, 4, 6, 7, 8, 9, 10, 11, 12)
Disease tissue studied: gastric cancer (3, 4), gastric carcinoma (3, 4)
Relevant cell line - cell type - tissue: HeLa (cervical) (2, 6, 7, 8, 9), Jurkat (T lymphocyte) (10, 11, 12), K562 (erythroid) (2), MKN-45 (gastric) (3, 4)




References

1

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

2

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

3

(2011) CST Curation Set: 12384; Year: 2011; SILAC: N; Biosample/Treatment: MKN-45(cell line)/untreated; Disease: gastric carcinoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995
Curated Info

4

(2011) CST Curation Set: 12386; Year: 2011; SILAC: N; Biosample/Treatment: MKN-45(cell line)/untreated; Disease: gastric carcinoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995
Curated Info

5

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

6

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

7

(2010) CST Curation Set: 10710; Year: 2010; SILAC: N; Biosample/Treatment: HeLa(cell line)/untreated; Disease: cervical adenocarcinoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-Thr-Pro Motif Antibody (polyAB) Cat#: 3003, PTMScan(R) Phospho-Thr-Pro Motif (T*P) Immunoaffinity Beads Cat#: 1996
Curated Info

8

(2010) CST Curation Set: 10689; Year: 2010; SILAC: N; Biosample/Treatment: HeLa(cell line)/nocodazole; Disease: cervical adenocarcinoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-Thr-Pro Motif Antibody (polyAB) Cat#: 3003, PTMScan(R) Phospho-Thr-Pro Motif (T*P) Immunoaffinity Beads Cat#: 1996
Curated Info

9

(2010) CST Curation Set: 10686; Year: 2010; SILAC: N; Biosample/Treatment: HeLa(cell line)/untreated; Disease: cervical adenocarcinoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-Thr-Pro Motif Antibody (polyAB) Cat#: 3003, PTMScan(R) Phospho-Thr-Pro Motif (T*P) Immunoaffinity Beads Cat#: 1996
Curated Info

10

(2009) CST Curation Set: 6852; Year: 2009; SILAC: N; Biosample/Treatment: Jurkat(cell line)/pervanadate & calyculin; Disease: T cell leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-Thr-Pro Motif Antibody (polyAB) Cat#: 3003, PTMScan(R) Phospho-Thr-Pro Motif (T*P) Immunoaffinity Beads Cat#: 1996
Curated Info

11

(2009) CST Curation Set: 6851; Year: 2009; SILAC: N; Biosample/Treatment: Jurkat(cell line)/pervanadate & calyculin; Disease: T cell leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

12

(2009) CST Curation Set: 6850; Year: 2009; SILAC: N; Biosample/Treatment: Jurkat(cell line)/pervanadate & calyculin; Disease: T cell leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

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