Tyr69
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Phosphorylation Site Page:
Tyr69 - DDX3 (mouse)

Site Information
SSKDKDAySSFGSRG    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: DDX3 (human): Y69, DDX3 (rat): Y69
Blast this site against: NCBI  SwissProt  PDB 
Available spectra:  2 CST

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (1, 6, 11, 16, 17, 18, 19, 20, 21, 23, 24)
Relevant cell line - cell type - tissue: 3T3 (fibroblast) (21, 22, 23), 3T3 (fibroblast) [Pyk2 (human), transfection] (1), 3T3-L1 (fibroblast) (17), BaF3 ('B lymphocyte, precursor') (6, 18, 19, 20, 24), MEF (fibroblast) [Src (mouse)] (11)




References

1

Bonnette PC, et al. (2010) Phosphoproteomic characterization of PYK2 signaling pathways involved in osteogenesis. J Proteomics 73, 1306-20
20116462   Curated Info

2

(2010) CST Curation Set: 9104; Year: 2010; SILAC: N; Biosample/Treatment: ES J1(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-102) Cat#: 9416
Curated Info

3

(2010) CST Curation Set: 9109; Year: 2010; SILAC: N; Biosample/Treatment: P19(cell line)/untreated; Disease: testicular cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-102) Cat#: 9416
Curated Info

4

(2010) CST Curation Set: 9108; Year: 2010; SILAC: N; Biosample/Treatment: PY2(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-102) Cat#: 9416
Curated Info

5

(2009) CST Curation Set: 8088; Year: 2009; SILAC: N; Biosample/Treatment: embryonic stem cells (mouse)(cell line)(PTEN)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

(2009) CST Curation Set: 7223; Year: 2009; SILAC: N; Biosample/Treatment: BaF3(cell line)/serum starved; Disease: chronic myelogenous leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

(2009) CST Curation Set: 7168; Year: 2009; SILAC: N; Biosample/Treatment: RAW 264.7(cell line)/LPS; Disease: leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

(2009) CST Curation Set: 7169; Year: 2009; SILAC: N; Biosample/Treatment: RAW 264.7(cell line)/MG132; Disease: leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

(2009) CST Curation Set: 7166; Year: 2009; SILAC: N; Biosample/Treatment: RAW 264.7(cell line)/control; Disease: leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

(2008) CST Curation Set: 4936; Year: 2008; SILAC: N; Biosample/Treatment: C2C12(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

11

Luo W, et al. (2008) Global impact of oncogenic Src on a phosphotyrosine proteome. J Proteome Res 7, 3447-60
18563927   Curated Info

12

(2008) CST Curation Set: 4438; Year: 2008; SILAC: N; Biosample/Treatment: lung(tissue)/untreated; Disease: non-small cell lung cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

13

(2008) CST Curation Set: 4440; Year: 2008; SILAC: N; Biosample/Treatment: lung(tissue)/untreated; Disease: non-small cell lung cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

14

(2007) CST Curation Set: 2564; Year: 2007; SILAC: N; Biosample/Treatment: C2C12-D(cell line)/-; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

15

(2007) CST Curation Set: 2565; Year: 2007; SILAC: N; Biosample/Treatment: C2C12-D(cell line)/Insulin; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

16

(2006) CST Curation Set: 1969; Year: 2006; SILAC: Y; Biosample1/Treatment/Isotope: brain(tissue)/untreated/L, Biosample2/Treatment/Isotope: brain(tissue)/untreated/H; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-YX(s/t)(phosphorylation)
Curated Info

17

Schmelzle K, et al. (2006) Temporal dynamics of tyrosine phosphorylation in insulin signaling. Diabetes 55, 2171-9
16873679   Curated Info

18

(2006) CST Curation Set: 1316; Year: 2006; SILAC: N; Biosample/Treatment: BaF3(cell line)/-; Disease: chronic myelogenous leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

19

(2005) CST Curation Set: 1056; Year: 2005; SILAC: N; Biosample/Treatment: BaF3(cell line)/-; Disease: chronic myelogenous leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

20

(2005) CST Curation Set: 860; Year: 2005; SILAC: N; Biosample/Treatment: BaF3(cell line)/-; Disease: chronic myelogenous leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

21

(2005) CST Curation Set: 630; Year: 2005; SILAC: N; Biosample/Treatment: 3T3(cell line)/EGF; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

22

(2003) CST Curation Set: 150; Year: 2003; SILAC: N; Biosample/Treatment: 3T3(cell line)/-; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

23

(2003) CST Curation Set: 90; Year: 2003; SILAC: N; Biosample/Treatment: 3T3(cell line)/-; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

24

(2003) CST Curation Set: 33; Year: 2003; SILAC: N; Biosample/Treatment: BaF3(cell line)/pervanadate & Flt3 ligand; Disease: chronic myelogenous leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

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