Ser369
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Phosphorylation Site Page:
Ser369 - p90RSK (mouse)

Site Information
HQLFRGFsFVATGLM    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: p90RSK (rat): S380, p90RSK (chicken): S398, p90RSK (human): S380, p90RSK iso3 (human): S389, p90RSK iso3 (mouse):
Blast this site against: NCBI  SwissProt  PDB 

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (2, 3, 4, 20, 21, 22, 24), phospho-antibody (1, 19, 23, 25, 26, 27, 28, 29, 30), western blotting (1, 19, 23, 27, 28)
Disease tissue studied: melanoma skin cancer (21)
Relevant cell line - cell type - tissue: 'fat, brown'-'fat, brown' (2), 'neuron, hippocampal'-brain [neurogranin (mouse)] (28), 293 (epithelial) (19), 32D (myeloid) (27), 3T3 (fibroblast) (23), BaF3 ('B lymphocyte, precursor') (25, 27), brain (2, 4), dendritic cell (23), dendritic cell [MAPKAPK2 (mouse), homozygous knockout] (23), dendritic cell [MSK1 (mouse), homozygous knockout] (23), E14 ('neuron, hippocampal') (1), epithelial (26), heart (2, 19), Hepa 1-6 (epithelial) (22), hepatic stellate (29), JB (epithelial) (30), kidney (2), liver (2, 24), lung (2), macrophage-bone marrow (3), macrophage-bone marrow [MKP-1 (mouse), homozygous knockout] (3), MC/9 (mast) (27), MEF (fibroblast) (30), myocyte-heart (19), RAW 267.4 (macrophage) (20), skin [mGluR1 (mouse), transgenic, TG mutant mice] (21), spleen (2)

Controlled by
Regulatory protein: MAPKAPK2 (mouse) (23), MAPKAPK3 (mouse) (23), neurogranin (mouse) (28)
Putative upstream kinases: ERK1 (mouse) (1), ERK2 (mouse) (1)
Kinases, in vitro: MAPKAPK2 (human) (23)
Treatments: AG1296 (25), anisomycin (23), BIRB0796 (23), CCL4 (29), EGF (23, 30), forskolin (28), IL-4 (27), IL-9 (27), LPS (3, 23), Pam3CSK4 (23), PD169316 (30), PD184352 (23), PD98059 (30), phorbol ester (23, 28, 30), SB202190 (30), SB203347 (23), SB203580 (23), SB203680 (23), TCAP-1 (1), TNF (23), U0126 (1, 25), UV (30)

Downstream Regulation
Effects of modification on p90RSK: enzymatic activity, induced (25)


References

1

Chand D, et al. (2012) C-Terminal region of teneurin-1 co-localizes with dystroglycan and modulates cytoskeletal organization through an extracellular signal-regulated kinase-dependent stathmin- and filamin A-mediated mechanism in hippocampal cells. Neuroscience 219, 255-70
22698694   Curated Info

2

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

3

Weintz G, et al. (2010) The phosphoproteome of toll-like receptor-activated macrophages. Mol Syst Biol 6, 371
20531401   Curated Info

4

Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9
20415495   Curated Info

5

(2009) CST Curation Set: 8606; Year: 2009; Biosample: embryonic stem cells (mouse)(PTEN); Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

6

(2009) CST Curation Set: 8601; Year: 2009; Biosample: embryonic stem cells (mouse)(PTEN); Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

7

(2009) CST Curation Set: 8604; Year: 2009; Biosample: embryonic stem cells (mouse)(PTEN); Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

8

(2009) CST Curation Set: 8603; Year: 2009; Biosample: embryonic stem cells (mouse)(PTEN); Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

9

(2009) CST Curation Set: 8605; Year: 2009; Biosample: embryonic stem cells (mouse)(PTEN); Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

10

(2009) CST Curation Set: 8607; Year: 2009; Biosample: embryonic stem cells (mouse)(PTEN); Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

11

(2009) CST Curation Set: 8602; Year: 2009; Biosample: embryonic stem cells (mouse)(PTEN); Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

12

(2009) CST Curation Set: 8600; Year: 2009; Biosample: embryonic stem cells (mouse)(PTEN); Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

13

(2009) CST Curation Set: 7446; Year: 2009; Biosample: liver; Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

14

(2009) CST Curation Set: 7447; Year: 2009; Biosample: mouse liver; Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

15

(2009) CST Curation Set: 7441; Year: 2009; Biosample: brain, embryonic; Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

16

(2009) CST Curation Set: 7443; Year: 2009; Biosample: brain; Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

17

(2009) CST Curation Set: 7440; Year: 2009; Biosample: brain, embryonic; Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

18

(2009) CST Curation Set: 7442; Year: 2009; Biosample: brain; Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

19

Lorenz K, Schmitt JP, Schmitteckert EM, Lohse MJ (2009) A new type of ERK1/2 autophosphorylation causes cardiac hypertrophy. Nat Med 15, 75-83
19060905   Curated Info

20

Trost M, et al. (2009) The phagosomal proteome in interferon-gamma-activated macrophages. Immunity 30, 143-54
19144319   Curated Info

21

Zanivan S, et al. (2008) Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-26
19367708   Curated Info

22

Pan C, Gnad F, Olsen JV, Mann M (2008) Quantitative phosphoproteome analysis of a mouse liver cell line reveals specificity of phosphatase inhibitors. Proteomics 8, 4534-46
18846507   Curated Info

23

Zaru R, et al. (2007) The MAPK-activated kinase Rsk controls an acute Toll-like receptor signaling response in dendritic cells and is activated through two distinct pathways. Nat Immunol 8, 1227-35
17906627   Curated Info

24

Villén J, Beausoleil SA, Gerber SA, Gygi SP (2007) Large-scale phosphorylation analysis of mouse liver. Proc Natl Acad Sci U S A 104, 1488-93
17242355   Curated Info

25

Yang X, et al. (2005) The FLT3 Internal tandem duplication mutation prevents apoptosis in interleukin-3-deprived BaF3 cells due to protein kinase A and ribosomal S6 kinase 1-mediated BAD phosphorylation at serine 112. Cancer Res 65, 7338-47
16103085   Curated Info

26

Miyagawa S, Katsu Y, Watanabe H, Iguchi T (2004) Estrogen-independent activation of erbBs signaling and estrogen receptor alpha in the mouse vagina exposed neonatally to diethylstilbestrol. Oncogene 23, 340-9
14647453   Curated Info

27

Demoulin JB, et al. (2003) MAP kinase activation by interleukin-9 in lymphoid and mast cell lines. Oncogene 22, 1763-70
12660812   Curated Info

28

Wu J, Li J, Huang KP, Huang FL (2002) Attenuation of protein kinase C and cAMP-dependent protein kinase signal transduction in the neurogranin knockout mouse. J Biol Chem 277, 19498-505
11912190   Curated Info

29

Buck M, Poli V, Hunter T, Chojkier M (2001) C/EBPbeta phosphorylation by RSK creates a functional XEXD caspase inhibitory box critical for cell survival. Mol Cell 8, 807-16
11684016   Curated Info

30

Zhang Y, et al. (2001) UVA induces Ser381 phosphorylation of p90RSK/MAPKAP-K1 via ERK and JNK pathways. J Biol Chem 276, 14572-80
11278279   Curated Info

Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.