Tyr1180

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Phosphorylation Site Page:

Tyr1180 - InsR (mouse)

Site Information

DIYETDYyRKGGKGL SwissProt Entrez-Gene

Predicted information: Scansite

Orthologous residues: InsR (human): Y1190, InsR iso2 (human): Y1178, InsR (rat): Y1191

Blast this site against: NCBI SwissProt PDB

In vivo Characterization

Methods used to characterize site in vivo:	mass spectrometry (1, 5, 17), phospho-antibody (2, 7, 18, 19, 22), western blotting (2, 7, 19, 22)
Relevant cell line - cell type - tissue:	'brain, cerebral cortex' (22), 'muscle, skeletal' (7), 'stem, embryonic' (7), 3T3 (fibroblast) [Pyk2 (human), transfection] (1), 3T3-L1 (fibroblast) (17), 831/13 (20, 21), adipose tissue (7), heart (7), hepatocyte-liver (2), liver (2, 7, 18), MEF (fibroblast) (5), MEF (fibroblast) [IGF1R (mouse)] (19)

Controlled by

Regulatory protein:	LPIN1 (mouse) (2), PDK1 (mouse) (7)
Putative upstream phosphatases:	PTP1B (mouse) (18)
Treatments:	insulin (7, 18, 19), siRNA (19)

Disease / Diagnostics Relevance

Relevant diseases:

type 2 diabetes (22)

	References

1	Bonnette PC, et al. (2010) Phosphoproteomic characterization of PYK2 signaling pathways involved in osteogenesis. J Proteomics 73, 1306-20 20116462 Curated Info
2	Ryu D, et al. (2009) TORC2 regulates hepatic insulin signaling via a mammalian phosphatidic acid phosphatase, LIPIN1. Cell Metab 9, 240-51 19254569 Curated Info
3	(2008) CST Curation Set: 5665; Year: 2008; Biosample: heart; Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
4	(2008) CST Curation Set: 4985; Year: 2008; Biosample: lung; Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
5	Luo W, et al. (2008) Global impact of oncogenic Src on a phosphotyrosine proteome. J Proteome Res 7, 3447-60 18563927 Curated Info
6	(2008) CST Curation Set: 4438; Year: 2008; Biosample: lung; Disease: non-small cell lung cancer; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
7	Bayascas JR, et al. (2008) Mutation of the PDK1 PH domain inhibits protein kinase B/Akt, leading to small size and insulin resistance. Mol Cell Biol 28, 3258-72 18347057 Curated Info
8	(2007) CST Curation Set: 3379; Year: 2007; Biosample: Mouse Mesenchymal Stem Cells; Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
9	(2007) CST Curation Set: 3380; Year: 2007; Biosample: Mouse Mesenchymal Stem Cells; Disease: -; Treatment: bFGF; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
10	(2007) CST Curation Set: 2989; Year: 2007; Biosample: liver; Disease: -; Treatment: Insulin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
11	(2007) CST Curation Set: 2990; Year: 2007; Biosample: liver; Disease: -; Treatment: Insulin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
12	(2007) CST Curation Set: 2985; Year: 2007; Biosample: liver; Disease: -; Treatment: Insulin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
13	(2007) CST Curation Set: 2986; Year: 2007; Biosample: liver; Disease: -; Treatment: Insulin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
14	(2007) CST Curation Set: 2827; Year: 2007; Biosample: heart; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
15	(2007) CST Curation Set: 2830; Year: 2007; Biosample: liver; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
16	(2007) CST Curation Set: 2565; Year: 2007; Biosample: C2C12-D; Disease: -; Treatment: Insulin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
17	Schmelzle K, et al. (2006) Temporal dynamics of tyrosine phosphorylation in insulin signaling. Diabetes 55, 2171-9 16873679 Curated Info
18	Haj FG, et al. (2005) Liver-specific protein-tyrosine phosphatase 1B (PTP1B) re-expression alters glucose homeostasis of PTP1B-/-mice. J Biol Chem 280, 15038-46 15699041 Curated Info
19	Galic S, et al. (2005) Coordinated regulation of insulin signaling by the protein tyrosine phosphatases PTP1B and TCPTP. Mol Cell Biol 25, 819-29 15632081 Curated Info
20	(2004) CST Curation Set: 380; Year: 2004; Biosample: 831/13; Disease: -; Treatment: Serum starved; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
21	(2004) CST Curation Set: 381; Year: 2004; Biosample: 831/13; Disease: -; Treatment: Serum starved & IGF; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
22	Ho L, et al. (2004) Diet-induced insulin resistance promotes amyloidosis in a transgenic mouse model of Alzheimer's disease. FASEB J 18, 902-4 15033922 Curated Info

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