Tyr809

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Phosphorylation Site Page:

Tyr809 - CSFR (human)

Site Information

DIMNDSNyIVKGNAR SwissProt Entrez-Gene

Predicted information: Scansite

Orthologous residues: CSFR (mouse): Y807

Blast this site against: NCBI SwissProt PDB

Available spectra: 2 CST

In vivo Characterization

Methods used to characterize site in vivo:	mass spectrometry (1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14), mutation of modification site (15, 16, 17, 19, 20, 21), phospho-antibody (15, 16, 20), western blotting (15, 16, 17)
Disease tissue studied:	breast cancer (7, 8, 11, 12, 13, 14), leukemia (5, 6), acute myelogenous leukemia (5, 6), lung cancer (2, 9, 10), non-small cell lung cancer (2, 9, 10)
Relevant cell line - cell type - tissue:	32D (myeloid) (15), 3T3 (fibroblast) (16, 17, 19), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] (20), FDCP-1 (myeloid) (21), GDM-1 (myeloid) (6), Jurkat (T lymphocyte) (3), lung (2, 9, 10), MCF-10A (breast cell) (1, 7, 8, 11, 12, 13, 14), MCF-10A (breast cell) [CSFR (human), transfection] (1), NCI-H2444 (pulmonary) (2), NKM-1 (myeloid) (5)

Controlled by

Regulatory protein:	CSFR (human) (1)
Putative upstream kinases:	CSFR (human) (15)
Kinases, in vitro:	CSFR (human) (21)
Treatments:	CSF-1 (15, 20), SU6656 (15)

Downstream Regulation

Effects of modification on CSFR:	activation (15, 21), phosphorylation (16)
Effects of modification on biological processes:	cell cycle regulation (21), cell growth, altered (19), transcription, altered (16)

	References

1	Knowlton ML, et al. (2010) Profiling Y561-Dependent and -Independent Substrates of CSF-1R in Epithelial Cells. PLoS One 5, e13587 21049007 Curated Info
2	Rikova K, et al. (2007) Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. Cell 131, 1190-203 18083107 Curated Info
3	(2007) CST Curation Set: 3519; Year: 2007; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
4	Schubert C, et al. (2007) Crystal structure of the tyrosine kinase domain of colony-stimulating factor-1 receptor (cFMS) in complex with two inhibitors. J Biol Chem 282, 4094-101 17132624 Curated Info
5	(2005) CST Curation Set: 818; Year: 2005; Biosample: NKM-1; Disease: leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
6	(2005) CST Curation Set: 893; Year: 2005; Biosample: GDM-1; Disease: acute myelogenous leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
7	(2005) CST Curation Set: 752; Year: 2005; Biosample: MCF-10A; Disease: breast cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
8	(2005) CST Curation Set: 753; Year: 2005; Biosample: MCF-10A; Disease: breast cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
9	(2005) CST Curation Set: 746; Year: 2005; Biosample: lung; Disease: non-small cell lung cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
10	(2005) CST Curation Set: 692; Year: 2005; Biosample: lung; Disease: non-small cell lung cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
11	(2005) CST Curation Set: 583; Year: 2005; Biosample: MCF-10A; Disease: breast cancer; Treatment: su 6656; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
12	(2005) CST Curation Set: 584; Year: 2005; Biosample: MCF-10A; Disease: breast cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
13	(2004) CST Curation Set: 498; Year: 2004; Biosample: MCF-10A; Disease: breast cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
14	(2004) CST Curation Set: 499; Year: 2004; Biosample: MCF-10A; Disease: breast cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
15	Rohde CM, Schrum J, Lee AW (2004) A juxtamembrane tyrosine in the colony stimulating factor-1 receptor regulates ligand-induced Src association, receptor kinase function, and down-regulation. J Biol Chem 279, 43448-61 15297464 Curated Info
16	Ezashi T, Roberts RM (2004) Regulation of interferon-tau (IFN-tau) gene promoters by growth factors that target the Ets-2 composite enhancer: a possible model for maternal control of IFN-tau production by the conceptus during early pregnancy. Endocrinology 145, 4452-60 15217985 Curated Info
17	Novak U, Nice E, Hamilton JA, Paradiso L (1996) Requirement for Y706 of the murine (or Y708 of the human) CSF-1 receptor for STAT1 activation in response to CSF-1. Oncogene 13, 2607-13 9000134 Curated Info
18	Joos H, et al. (1996) Tyrosine phosphorylation of the juxtamembrane domain of the v-Fms oncogene product is required for its association with a 55-kDa protein. J Biol Chem 271, 24476-81 8798707 Curated Info
19	Rabault B, Roussel MF, Quang CT, Ghysdael J (1996) Phosphorylation of Ets1 regulates the complementation of a CSF-1 receptor impaired in mitogenesis. Oncogene 13, 877-81 8761310 Curated Info
20	Novak U, et al. (1995) Colony-stimulating factor 1-induced STAT1 and STAT3 activation is accompanied by phosphorylation of Tyk2 in macrophages and Tyk2 and JAK1 in fibroblasts. Blood 86, 2948-56 7579387 Curated Info
21	Baker DA, Maher J, Roberts IA, Dibb NJ (1994) Evidence that ras and myc mediate the synergy between SCF or M-CSF and other haemopoietic growth factors. Leukemia 8, 1970-81 7526094 Curated Info

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