Ser126
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Phosphorylation Site Page:
Ser126 - Pnk1 (human)

Site Information
PPGTPLVsQDEKRDA    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: Pnk1 (mouse): P126, Pnk1 (rat): P126
Blast this site against: NCBI  SwissProt  PDB 

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
Disease tissue studied: brain cancer (11, 23, 24), glioblastoma (11, 23, 24), glioma (11, 23, 24), Seckel syndrome (11)
Relevant cell line - cell type - tissue: 293 (epithelial) (6), 293 (epithelial) [AT1 (human), transfection] (5), 293T (epithelial) (12), GM00200 (fibroblast) (11, 14, 15), GM18366 (fibroblast) (11), HeLa (cervical) (2, 4), Jurkat (T lymphocyte) (7, 8, 9), K562 (erythroid) (10), M059J (glial) (22, 24), M059K (glial) (11, 13, 16, 17, 18, 19, 20, 21, 23)

Controlled by
Treatments: UV (11)



References

1

Zolner AE, et al. (2011) Phosphorylation of polynucleotide kinase/ phosphatase by DNA-dependent protein kinase and ataxia-telangiectasia mutated regulates its association with sites of DNA damage. Nucleic Acids Res 39, 9224-37
21824916   Curated Info

2

(2011) CST Curation Set: 11708; Year: 2011; SILAC: N; Biosample/Treatment: HeLa(cell line)/UV; Disease: cervical adenocarcinoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate (S*/T*QG) (P-S/T2-100) Rabbit mAb Cat#: 6966, PTMScan(R) Phospho-ATM/ATR Substrate Motif (S*/T*QG) Immunoaffinity Beads Cat#: 6969
Curated Info

3

Segal-Raz H, et al. (2011) ATM-mediated phosphorylation of polynucleotide kinase/phosphatase is required for effective DNA double-strand break repair. EMBO Rep 12, 713-9
21637298   Curated Info

4

(2010) CST Curation Set: 10689; Year: 2010; SILAC: N; Biosample/Treatment: HeLa(cell line)/nocodazole; Disease: cervical adenocarcinoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-Thr-Pro Motif Antibody (polyAB) Cat#: 3003, PTMScan(R) Phospho-Thr-Pro Motif (T*P) Immunoaffinity Beads Cat#: 1996
Curated Info

5

Xiao K, et al. (2010) Global phosphorylation analysis of beta-arrestin-mediated signaling downstream of a seven transmembrane receptor (7TMR). Proc Natl Acad Sci U S A 107, 15299-304
20686112   Curated Info

6

(2010) CST Curation Set: 9972; Year: 2010; SILAC: N; Biosample/Treatment: 293(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Millipore 05-368
Curated Info

7

(2010) CST Curation Set: 9440; Year: 2010; SILAC: N; Biosample/Treatment: Jurkat(cell line)/pervanadate & calyculin; Disease: T cell leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

8

(2010) CST Curation Set: 9439; Year: 2010; SILAC: N; Biosample/Treatment: Jurkat(cell line)/pervanadate & calyculin; Disease: T cell leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) Antibody Used to Purify Peptides prior to MS2: T*PP Motif (T*PP) (D61C3) Rabbit mAb Cat#: 5757, PTMScan(R) T*PP Motif (T*PP) Immunoaffinity Beads Cat#: 5758
Curated Info

9

(2009) CST Curation Set: 6850; Year: 2009; SILAC: N; Biosample/Treatment: Jurkat(cell line)/pervanadate & calyculin; Disease: T cell leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

10

(2008) CST Curation Set: 4609; Year: 2008; SILAC: N; Biosample/Treatment: K562(cell line)/untreated; Disease: chronic myelogenous leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-[sty](phosphorylation)
Curated Info

11

Stokes MP, et al. (2007) Profiling of UV-induced ATM/ATR signaling pathways. Proc Natl Acad Sci U S A 104, 19855-60
18077418   Curated Info

12

Matsuoka S, et al. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science 316, 1160-6
17525332   Curated Info

13

(2006) CST Curation Set: 1954; Year: 2006; SILAC: Y; Biosample1/Treatment/Isotope: M059K(cell line)/normal/L, Biosample2/Treatment/Isotope: M059K(cell line)/UV/H; Disease: glioblastoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

14

(2006) CST Curation Set: 1772; Year: 2006; SILAC: N; Biosample/Treatment: GM00200(cell line)/UV; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

15

(2006) CST Curation Set: 1773; Year: 2006; SILAC: N; Biosample/Treatment: GM00200(cell line)/UV; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

16

(2006) CST Curation Set: 1614; Year: 2006; SILAC: N; Biosample/Treatment: M059K(cell line)/normal; Disease: glioblastoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

17

(2006) CST Curation Set: 1616; Year: 2006; SILAC: N; Biosample/Treatment: M059K(cell line)/UV; Disease: glioblastoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

18

(2006) CST Curation Set: 1617; Year: 2006; SILAC: N; Biosample/Treatment: M059K(cell line)/UV; Disease: glioblastoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

19

(2006) CST Curation Set: 1122; Year: 2006; SILAC: N; Biosample/Treatment: M059K(cell line)/UV; Disease: glioblastoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

20

(2006) CST Curation Set: 1123; Year: 2006; SILAC: N; Biosample/Treatment: M059K(cell line)/UV; Disease: glioblastoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

21

(2006) CST Curation Set: 1091; Year: 2006; SILAC: N; Biosample/Treatment: M059K(cell line)/UV; Disease: glioblastoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

22

(2006) CST Curation Set: 1092; Year: 2006; SILAC: N; Biosample/Treatment: M059J(cell line)/UV; Disease: glioblastoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

23

(2005) CST Curation Set: 909; Year: 2005; SILAC: N; Biosample/Treatment: M059K(cell line)/UV; Disease: glioblastoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

24

(2005) CST Curation Set: 908; Year: 2005; SILAC: N; Biosample/Treatment: M059J(cell line)/UV; Disease: glioblastoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

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