Ser11
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Phosphorylation Site Page:
Ser11 - dCK (human)

Site Information
PPKRSCPsFSASSEG    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: dCK (mouse): S11, dCK (rat): S11
Blast this site against: NCBI  SwissProt  PDB 

In vivo Characterization
Methods used to characterize site in vivo: [32P] bio-synthetic labeling (13, 14), mass spectrometry (1, 2, 3, 4, 5, 6, 8, 10, 11, 12, 13, 14), mass spectrometry (in vitro) (7), mutation of modification site (9), phosphopeptide mapping (14)
Relevant cell line - cell type - tissue: 293T (epithelial) (9, 13, 14), A498 (renal) (8), HCT116 (intestinal) (10), HeLa (cervical) (1), HeLa S3 (cervical) (11), Jurkat (T lymphocyte) (2, 3, 4, 5, 6, 12), MV4-11 (macrophage) (10)

Controlled by
Kinases, in vitro: CK1D (human) (7)

Downstream Regulation
Effects of modification on dCK: phosphorylation (9)


References

1

Sharma K, et al. (2014) Ultradeep Human Phosphoproteome Reveals a Distinct Regulatory Nature of Tyr and Ser/Thr-Based Signaling. Cell Rep
25159151   Curated Info

2

(2012) CST Curation Set: 13109; Year: 2012; Biosample/Treatment: cell line, Jurkat/calyculin & pervanadate; Disease: T cell leukemia; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-[s/t/y](phosphorylation)
Curated Info

3

(2011) CST Curation Set: 12713; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin & pervanadate; Disease: T cell leukemia; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)XP(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser) 14-3-3 Binding Motif Antibody Cat#: 9601
Curated Info

4

(2011) CST Curation Set: 12074; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin & pervanadate; Disease: T cell leukemia; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(F/Y/M)X(s/t)(L/I/M)(phosphorylation)
Curated Info

5

(2011) CST Curation Set: 11989; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin & pervanadate; Disease: T cell leukemia; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)XP(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser) 14-3-3 Binding Motif Antibody Cat#: 9601
Curated Info

6

(2011) CST Curation Set: 11893; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin & pervanadate; Disease: T cell leukemia; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-[s/t/y](phosphorylation)
Curated Info

7

Smal C, et al. (2010) Casein kinase 1delta activates human recombinant deoxycytidine kinase by Ser-74 phosphorylation, but is not involved in the in vivo regulation of its activity. Arch Biochem Biophys 502, 44-52
20637175   Curated Info

8

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
20071362   Curated Info

9

Smal C, et al. (2010) Influence of phosphorylation of THR-3, SER-11, and SER-15 on deoxycytidine kinase activity and stability. Nucleosides Nucleotides Nucleic Acids 29, 404-7
20544527   Curated Info

10

Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64
19369195   Curated Info

11

Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48
18691976   Curated Info

12

(2008) CST Curation Set: 3884; Year: 2008; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-[sty](phosphorylation)
Curated Info

13

Smal C, et al. (2006) Identification of in vivo phosphorylation sites on human deoxycytidine kinase. Role of Ser-74 in the control of enzyme activity. J Biol Chem 281, 4887-93
16361699   Curated Info

14

Smal C, et al. (2006) Identification of phosphorylation sites on human deoxycytidine kinase after overexpression in eucaryotic cells. Nucleosides Nucleotides Nucleic Acids 25, 1141-6
17065079   Curated Info

Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.