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In vivo Characterization |
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| Methods used to characterize site in vivo: |
[32P] bio-synthetic labeling (10, 11), mass spectrometry (1, 2, 3, 4, 7, 8, 9, 10, 11), mass spectrometry (in vitro) (5), mutation of modification site (6), phosphopeptide mapping (11)
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| Relevant cell line - cell type - tissue: |
293T (epithelial) (6, 10, 11), HCT116 (intestinal) (7), HeLa S3 (cervical) (8), Jurkat (T lymphocyte) (1, 2, 3, 4, 9), MV4-11 (myeloid) (7)
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1
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(2011) CST Curation Set: 12713; Year: ; Biosample: Jurkat; Disease: T cell leukemia; Treatment: calyculin & pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)XP(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser) 14-3-3 Binding Motif Antibody Cat#: 9601
Curated Info
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2
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(2011) CST Curation Set: 12074; Year: 2011; Biosample: Jurkat; Disease: T cell leukemia; Treatment: calyculin & pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(F/Y/M)X(s/t)(L/I/M)(phosphorylation)
Curated Info
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3
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(2011) CST Curation Set: 11989; Year: 2011; Biosample: Jurkat; Disease: T cell leukemia; Treatment: calyculin & pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)XP(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser) 14-3-3 Binding Motif Antibody Cat#: 9601
Curated Info
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4
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(2011) CST Curation Set: 11893; Year: 2011; Biosample: Jurkat; Disease: T cell leukemia; Treatment: calyculin & pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-[s/t/y](phosphorylation)
Curated Info
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5
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Smal C, et al. (2010) Casein kinase 1delta activates human recombinant deoxycytidine kinase by Ser-74 phosphorylation, but is not involved in the in vivo regulation of its activity. Arch Biochem Biophys 502, 44-52
20637175
Curated Info
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6
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Smal C, et al. (2010) Influence of phosphorylation of THR-3, SER-11, and SER-15 on deoxycytidine kinase activity and stability. Nucleosides Nucleotides Nucleic Acids 29, 404-7
20544527
Curated Info
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7
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Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64
19369195
Curated Info
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8
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Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48
18691976
Curated Info
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9
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(2008) CST Curation Set: 3884; Year: 2008; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-[sty](phosphorylation)
Curated Info
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10
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Smal C, et al. (2006) Identification of in vivo phosphorylation sites on human deoxycytidine kinase. Role of Ser-74 in the control of enzyme activity. J Biol Chem 281, 4887-93
16361699
Curated Info
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11
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Smal C, et al. (2006) Identification of phosphorylation sites on human deoxycytidine kinase after overexpression in eucaryotic cells. Nucleosides Nucleotides Nucleic Acids 25, 1141-6
17065079
Curated Info
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