Tyr823
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Phosphorylation Site Page:
Tyr823 - Kit (human)

Site Information
DIKNDSNyVVKGNAR    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: Kit (mouse): Y825
Blast this site against: NCBI  SwissProt  PDB 
Available spectra:  2 CST

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (9, 10), mass spectrometry (in vitro) (4), mutation of modification site (11)
Disease tissue studied: leukemia (9, 10), acute myelogenous leukemia (9, 10)
Relevant cell line - cell type - tissue: CHRF (megakaryocyte) (9), IC2 (mast) (11), M-07e (myeloid) (10)

Controlled by
Kinases, in vitro: Kit (human) (4)



References

1

Agarwal S, Kazi JU, Rönnstrand L (2013) Phosphorylation of the Activation Loop Tyrosine 823 in c-Kit Is Crucial for Cell Survival and Proliferation. J Biol Chem 288, 22460-8
23803604   Curated Info

2

(2010) CST Curation Set: 10551; Year: 2010; SILAC: N; Biosample/Treatment: SKNBE(2)(cell line)/NGF; Disease: neuroblastoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

(2010) CST Curation Set: 10549; Year: 2010; SILAC: N; Biosample/Treatment: SKNBE(2)(cell line)/NGF; Disease: neuroblastoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

DiNitto JP, et al. (2010) Function of activation loop tyrosine phosphorylation in the mechanism of c-Kit auto-activation and its implication in sunitinib resistance. J Biochem 147, 601-9
20147452   Curated Info

5

(2009) CST Curation Set: 8060; Year: 2009; SILAC: N; Biosample/Treatment: gastrointestinal tract(tissue)/untreated; Disease: gastric cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

(2009) CST Curation Set: 7526; Year: 2009; SILAC: N; Biosample/Treatment: liver(tissue)/untreated; Disease: liver cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

(2008) CST Curation Set: 4091; Year: 2008; SILAC: N; Biosample/Treatment: gastrointestinal tract(tissue)/untreated; Disease: gastric cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

(2008) CST Curation Set: 3988; Year: 2008; SILAC: N; xenograft, Biosample/Treatment: gastrointestinal tract(tissue)/untreated; Disease: gastric carcinoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

(2005) CST Curation Set: 842; Year: 2005; SILAC: N; Biosample/Treatment: CHRF(cell line)/-; Disease: acute myelogenous leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

(2005) CST Curation Set: 723; Year: 2005; SILAC: N; Biosample/Treatment: M-07e(cell line)/-; Disease: acute myelogenous leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

11

Koma Y, et al. (2005) Distinct role for c-kit receptor tyrosine kinase and SgIGSF adhesion molecule in attachment of mast cells to fibroblasts. Lab Invest 85, 426-35
15654360   Curated Info

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