Tyr703
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Phosphorylation Site Page:
Tyr703 - Kit (human)

Site Information
DHAEAALyKNLLHSK    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: Kit (mouse): Y706, Kit (rat): Y705
Blast this site against: NCBI  SwissProt  PDB 
Available spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo: [32P] bio-synthetic labeling (24), mass spectrometry (1, 2, 3, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21, 22), mass spectrometry (in vitro) (5), mutation of modification site (4, 24), phospho-antibody (19, 23), phosphoamino acid analysis (24), western blotting (4, 19, 23)
Disease tissue studied: brain cancer (1, 2), astrocytoma (1, 2), breast cancer (13, 14), colorectal cancer (19), colorectal carcinoma (19), gastric cancer (6, 8, 9, 10, 19, 23), gastric carcinoma (8), GIST (19, 23), leukemia (11, 16, 17, 18, 20, 21, 22), acute myelogenous leukemia (11, 16, 17, 18, 20, 21, 22), liver cancer (7), small intestine cancer (23)
Relevant cell line - cell type - tissue: 293 (epithelial) (4), CHRF (megakaryocyte) (17), CMS (myeloid) (15, 16), GIST544 (gastric) (23), GIST882 (gastric) (19, 23), HCC1599 (breast cell) (13, 14), HCT116 (intestinal) (19), HEL (erythroid) (18), HES-3 ('stem, embryonic') (3), Kasumi-1 (myeloid) (20), liver (7), M-07e (myeloid) (21), NOMO-1 (myeloid) (11), PAE (endothelial) (24), stomach (6, 8, 9, 10), U-118MG (glial) (1, 2), UT-7 (myeloid) (22)

Controlled by
Kinases, in vitro: Kit (human) (5, 24)
Treatments: CSF-1 (24), DRB (19), flavopiridol (19), imatinib (19, 23), SCF (23)


Disease / Diagnostics Relevance
Relevant diseases: GIST (23)

References

1

(2011) CST Curation Set: 11915; Year: 2011; Biosample/Treatment: cell line, U118 MG/normal; Disease: astrocytoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

2

(2011) CST Curation Set: 11916; Year: 2011; Biosample/Treatment: cell line, U118 MG/normal; Disease: astrocytoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

Ding VM, et al. (2011) Tyrosine phosphorylation profiling in fgf-2 stimulated human embryonic stem cells. PLoS One 6, e17538
21437283   Curated Info

4

Phung B, et al. (2011) C-KIT signaling depends on microphthalmia-associated transcription factor for effects on cell proliferation. PLoS One 6, e24064
21887372   Curated Info

5

DiNitto JP, et al. (2010) Function of activation loop tyrosine phosphorylation in the mechanism of c-Kit auto-activation and its implication in sunitinib resistance. J Biochem 147, 601-9
20147452   Curated Info

6

(2009) CST Curation Set: 8060; Year: 2009; Biosample/Treatment: tissue, gastrointestinal tract/untreated; Disease: gastric cancer; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

(2009) CST Curation Set: 7526; Year: 2009; Biosample/Treatment: tissue, liver/untreated; Disease: liver cancer; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

(2008) CST Curation Set: 5604; Year: 2008; Biosample/Treatment: tissue, gastrointestinal tract/untreated; Disease: gastric carcinoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

(2008) CST Curation Set: 5501; Year: 2008; Biosample/Treatment: tissue, gastrointestinal tract/untreated; Disease: gastric cancer; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

(2008) CST Curation Set: 4091; Year: 2008; Biosample/Treatment: tissue, gastrointestinal tract/untreated; Disease: gastric cancer; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

11

(2007) CST Curation Set: 2523; Year: 2007; Biosample/Treatment: cell line, Nomo-1/DMSO; Disease: acute myelogenous leukemia; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

12

(2007) CST Curation Set: 2525; Year: 2007; Biosample/Treatment: cell line, Nomo-1/SAHA 3h; Disease: acute myelogenous leukemia; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

13

(2006) CST Curation Set: 2107; Year: 2006; Biosample/Treatment: cell line, HCC1599/0.5% serum; Disease: breast cancer; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

14

(2006) CST Curation Set: 2108; Year: 2006; Biosample/Treatment: cell line, HCC1599/0.5% serum; Disease: breast cancer; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

15

(2006) CST Curation Set: 1962; Year: 2006; Biosample/Treatment: cell line, CMS/untreated; Disease: acute myelogenous leukemia; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

16

(2006) CST Curation Set: 1875; Year: 2006; Biosample/Treatment: cell line, CMS/untreated; Disease: acute myelogenous leukemia; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

17

(2006) CST Curation Set: 1791; Year: 2006; Biosample/Treatment: cell line, CHRF/untreated; Disease: acute myelogenous leukemia; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

18

(2006) CST Curation Set: 1509; Year: 2006; Biosample/Treatment: cell line, HEL/untreated; Disease: erythroid leukemia; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

19

Sambol EB, et al. (2006) Flavopiridol targets c-KIT transcription and induces apoptosis in gastrointestinal stromal tumor cells. Cancer Res 66, 5858-66
16740725   Curated Info

20

(2006) CST Curation Set: 1196; Year: 2006; Biosample/Treatment: cell line, Kasumi-1/-; Disease: acute myelogenous leukemia; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

21

(2005) CST Curation Set: 723; Year: 2005; Biosample/Treatment: cell line, M-07e/-; Disease: acute myelogenous leukemia; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

22

(2005) CST Curation Set: 714; Year: 2005; Biosample/Treatment: cell line, UT-7/-; Disease: acute myelogenous leukemia; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

23

Duensing A, et al. (2004) Mechanisms of oncogenic KIT signal transduction in primary gastrointestinal stromal tumors (GISTs). Oncogene 23, 3999-4006
15007386   Curated Info

24

Thömmes K, Lennartsson J, Carlberg M, Rönnstrand L (1999) Identification of Tyr-703 and Tyr-936 as the primary association sites for Grb2 and Grb7 in the c-Kit/stem cell factor receptor. Biochem J 341 ( Pt 1), 211-6
10377264   Curated Info

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