Ser516
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Phosphorylation Site Page:
Ser516 - Tau (human)

Site Information
GDRSGYSsPGSPGTP    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: Tau (mouse): S491, Tau (rat): S510, Tau iso8 (human): S199, Tau iso3 (human): S105, Tau iso2 (human): S141, Tau iso6 (human): S141, Tau (cow): S206, Tau iso5 (human): S199, Tau iso3 (mouse): S188, Tau iso7 (mouse): S148, Tau iso7 (human): S170
Blast this site against: NCBI  SwissProt  PDB 

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18), phospho-antibody (13), western blotting (13)
Disease tissue studied: FTLD (7)
Relevant cell line - cell type - tissue: 'brain, cerebral cortex' (7), A498 (renal) (8), Jurkat (T lymphocyte) (1, 2, 3, 4, 5, 9, 10, 11, 12, 16, 17, 18), MKN-45 (gastric) (14, 15), neuron-brain (13)

Controlled by
Phosphatases, in vitro: PPP2CA (human) (19), PPP5C (human) (19)
Treatments: APV (13), glutamate (13), MK801 (13), NMDA (13), okadaic acid (13)



References

1

(2011) CST Curation Set: 12677; Year: 2011; SILAC: N; Biosample/Treatment: Jurkat(cell line)/calyculin & pervanadate; Disease: T cell leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(F/Y/M)X(s/t)(L/I/M)(phosphorylation)
Curated Info

2

(2011) CST Curation Set: 12681; Year: 2011; SILAC: N; Biosample/Treatment: Jurkat(cell line)/calyculin & pervanadate; Disease: T cell leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(F/Y/M)X(s/t)(L/I/M)(phosphorylation)
Curated Info

3

(2011) CST Curation Set: 12438; Year: 2011; SILAC: N; Biosample/Treatment: Jurkat(cell line)/calyculin & pervanadate; Disease: T cell leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser) CDKs Substrate Antibody Cat#: 2324, PTMScan(R) Phospho-CDK Substrate Motif (K/RS*PXK/R) Immunoaffinity Beads Cat#: 1981
Curated Info

4

(2011) CST Curation Set: 11986; Year: 2011; SILAC: N; Biosample/Treatment: Jurkat(cell line)/calyculin & pervanadate; Disease: T cell leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)XP(phosphorylation)
Curated Info

5

(2011) CST Curation Set: 11987; Year: 2011; SILAC: N; Biosample/Treatment: Jurkat(cell line)/calyculin & pervanadate; Disease: T cell leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)XP(phosphorylation)
Curated Info

6

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

7

Herskowitz JH, et al. (2010) Phosphoproteomic Analysis Reveals Site-Specific Changes in GFAP and NDRG2 Phosphorylation in Frontotemporal Lobar Degeneration. J Proteome Res 9, 6368-79
20886841   Curated Info

8

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
20071362   Curated Info

9

(2010) CST Curation Set: 9695; Year: 2010; SILAC: N; Biosample/Treatment: Jurkat(cell line)/pervanadate & calyculin; Disease: T cell leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-YX(s/t)(phosphorylation)
Curated Info

10

(2010) CST Curation Set: 9642; Year: 2010; SILAC: N; Biosample/Treatment: Jurkat(cell line)/pervanadate & calyculin; Disease: T cell leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-sPX(I/L/M/V)(phosphorylation)
Curated Info

11

(2009) CST Curation Set: 7652; Year: 2009; SILAC: N; Biosample/Treatment: Jurkat(cell line)/pervanadate & calyculin; Disease: T cell leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-YX(s/t)(phosphorylation)
Curated Info

12

Mayya V, et al. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal 2, ra46
19690332   Curated Info

13

Rahman A, et al. (2009) The excitotoxin quinolinic acid induces tau phosphorylation in human neurons. PLoS One 4, e6344
19623258   Curated Info

14

(2008) CST Curation Set: 5570; Year: 2008; SILAC: N; Biosample/Treatment: MKN-45(cell line)/Su11274; Disease: gastric carcinoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser) CDKs Substrate Antibody Cat#: 2324, PTMScan(R) Phospho-CDK Substrate Motif (K/RS*PXK/R) Immunoaffinity Beads Cat#: 1981
Curated Info

15

(2008) CST Curation Set: 5571; Year: 2008; SILAC: N; Biosample/Treatment: MKN-45(cell line)/Su11274; Disease: gastric carcinoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser) CDKs Substrate Antibody Cat#: 2324, PTMScan(R) Phospho-CDK Substrate Motif (K/RS*PXK/R) Immunoaffinity Beads Cat#: 1981
Curated Info

16

(2008) CST Curation Set: 4704; Year: 2008; SILAC: N; Biosample/Treatment: Jurkat(cell line)/calyculin & pervanadate; Disease: T cell leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser) CDKs Substrate Antibody Cat#: 2324, PTMScan(R) Phospho-CDK Substrate Motif (K/RS*PXK/R) Immunoaffinity Beads Cat#: 1981
Curated Info

17

(2008) CST Curation Set: 4347; Year: 2008; SILAC: N; Biosample/Treatment: Jurkat(cell line)/pervanadate & calyculin; Disease: T cell leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser) CDKs Substrate Antibody Cat#: 2324, Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381
Curated Info

18

(2008) CST Curation Set: 3882; Year: 2008; SILAC: N; Biosample/Treatment: Jurkat(cell line)/pervanadate; Disease: T cell leukemia; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-[sty](phosphorylation)
Curated Info

19

Liu F, et al. (2002) Aberrant glycosylation modulates phosphorylation of tau by protein kinase A and dephosphorylation of tau by protein phosphatase 2A and 5. Neuroscience 115, 829-37
12435421   Curated Info

Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.