Tyr1361
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Phosphorylation Site Page:
Tyr1361 - InsR (human)

Site Information
SYEEHIPyTHMNGGK    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: InsR iso2 (human): Y1349, InsR (rat): Y1362, InsR (mouse): Y1351
Blast this site against: NCBI  SwissProt  PDB 

In vivo Characterization
Methods used to characterize site in vivo: 2D analysis (21), immunoprecipitation (23), mass spectrometry (1, 2, 3, 4, 10, 11, 14, 15, 16), mutation of modification site (19, 21, 22, 23), phospho-antibody (19, 23, 24), phosphoamino acid analysis (21), phosphopeptide mapping (21), western blotting (19)
Disease tissue studied: gastric cancer (2, 3, 10, 14, 15), gastric carcinoma (2, 3, 10, 15), neuroblastoma (16)
Relevant cell line - cell type - tissue: CHO (fibroblast) (22), CHO (fibroblast) [EphB1 (human), transfection] (23), COS (fibroblast) (21), HCT116 (intestinal) (1), hepatocyte [InsR (mouse)] (19), HES-3 ('stem, embryonic') (4), IM95 (gastric) (14), KMS-11 (B lymphocyte) (11), LAN-1 (neural crest) (16), MEF (fibroblast) [InsR (human), transfection] (24), MKN-45 (gastric) (2, 3, 10, 15)

Controlled by
Kinases, in vitro: InsR (human) (17, 18, 20, 23, 25, 26)
Phosphatases, in vitro: PTPRA (rat) (25)
Treatments: insulin (19, 21)

Downstream Regulation
Effects of modification on InsR: enzymatic activity, induced (17, 18), protein conformation (17)
Effects of modification on biological processes: cell cycle regulation (22), cell growth, altered (24)


References

1

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

2

(2012) CST Curation Set: 13326; Year: 2012; Biosample/Treatment: cell line, MKN-45/untreated; Disease: gastric carcinoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

(2011) CST Curation Set: 12519; Year: 2011; Biosample/Treatment: cell line, MKN-45/normal; Disease: gastric carcinoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Ding VM, et al. (2011) Tyrosine phosphorylation profiling in fgf-2 stimulated human embryonic stem cells. PLoS One 6, e17538
21437283   Curated Info

5

(2010) CST Curation Set: 10743; Year: 2010; Biosample/Treatment: cell line, OVKATE/serum starved; Disease: ovarian cancer; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

(2010) CST Curation Set: 10551; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

(2010) CST Curation Set: 10549; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

(2010) CST Curation Set: 10550; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

(2010) CST Curation Set: 10548; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

(2010) CST Curation Set: 9924; Year: 2010; Biosample/Treatment: cell line, MKN-45/DMSO; Disease: gastric carcinoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-[sty](phosphorylation)
Curated Info

11

St-Germain JR, et al. (2009) Multiple myeloma phosphotyrosine proteomic profile associated with FGFR3 expression, ligand activation, and drug inhibition. Proc Natl Acad Sci U S A 106, 20127-32
19901323   Curated Info

12

(2009) CST Curation Set: 6681; Year: 2009; Biosample/Treatment: cell line, Dana Farber BEAS LKB/untreated; Disease: -; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

13

(2009) CST Curation Set: 6682; Year: 2009; Biosample/Treatment: cell line, Dana Farber BEAS LKB/untreated; Disease: -; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

14

(2008) CST Curation Set: 3917; Year: 2008; Biosample/Treatment: cell line, IM95/serum starved; Disease: gastric cancer; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

15

Guo A, et al. (2008) Signaling networks assembled by oncogenic EGFR and c-Met. Proc Natl Acad Sci U S A 105, 692-7
18180459   Curated Info

16

(2006) CST Curation Set: 2043; Year: 2006; Biosample/Treatment: cell line, LAN-1/serum starved; Disease: -; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

17

Tennagels N, Bergschneider E, Al-Hasani H, Klein HW (2000) Autophosphorylation of the two C-terminal tyrosine residues Tyr1316 and Tyr1322 modulates the activity of the insulin receptor kinase in vitro. FEBS Lett 479, 67-71
10940390   Curated Info

18

Noelle V, Tennagels N, Klein HW (2000) A single substitution of the insulin receptor kinase inhibits serine autophosphorylation in vitro: evidence for an interaction between the C-terminus and the activation loop. Biochemistry 39, 7170-7
10852715   Curated Info

19

Soni P, et al. (2000) The differential effects of pp120 (Ceacam 1) on the mitogenic action of insulin and insulin-like growth factor 1 are regulated by the nonconserved tyrosine 1316 in the insulin receptor. Mol Cell Biol 20, 3896-905
10805733   Curated Info

20

Al-Hasani H, et al. (1997) Identification of Ser-1275 and Ser-1309 as autophosphorylation sites of the insulin receptor. FEBS Lett 400, 65-70
9000514   Curated Info

21

Lewis RE, Volle DJ, Sanderson SD (1994) Phorbol ester stimulates phosphorylation on serine 1327 of the human insulin receptor. J Biol Chem 269, 26259-66
7929343   Curated Info

22

Ando A, et al. (1992) Enhanced insulin-induced mitogenesis and mitogen-activated protein kinase activities in mutant insulin receptors with substitution of two COOH-terminal tyrosine autophosphorylation sites by phenylalanine. J Biol Chem 267, 12788-96
1618780   Curated Info

23

Murakami MS, Rosen OM (1991) The role of insulin receptor autophosphorylation in signal transduction. J Biol Chem 266, 22653-60
1658004   Curated Info

24

Baron V, et al. (1991) The carboxyl-terminal domain of the insulin receptor: its potential role in growth-promoting effects. Biochemistry 30, 9365-70
1654097   Curated Info

25

King MJ, Sale GJ (1990) Dephosphorylation of insulin-receptor autophosphorylation sites by particulate and soluble phosphotyrosyl-protein phosphatases. Biochem J 266, 251-9
1689998   Curated Info

26

Tavaré JM, Denton RM (1988) Studies on the autophosphorylation of the insulin receptor from human placenta. Analysis of the sites phosphorylated by two-dimensional peptide mapping. Biochem J 252, 607-15
3166375   Curated Info

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