Javascript is not enabled on this browser. This site will not work properly without Javascript.
 |
In vivo Characterization |
 |
| Methods used to characterize site in vivo: |
2D analysis (9), immunoprecipitation (11), mass spectrometry (1, 2, 4), mutation of modification site (7, 9, 10, 11), phospho-antibody (7, 11, 12), phosphoamino acid analysis (9), phosphopeptide mapping (9), western blotting (7)
|
| Disease tissue studied: |
neuroblastoma (4)
|
| Relevant cell line - cell type - tissue: |
CHO (fibroblast) (10), CHO (fibroblast) [EphB1 (human), transfection] (11), COS (fibroblast) (9), HCT116 (intestinal) (1), hepatocyte [InsR (mouse)] (7), KMS-11 (B lymphocyte) (2), LAN-1 (neural crest) (4), MEF (fibroblast) [InsR (human), transfection] (12)
|
|
|
1
|
Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096
Curated Info
|
|
2
|
St-Germain JR, et al. (2009) Multiple myeloma phosphotyrosine proteomic profile associated with FGFR3 expression, ligand activation, and drug inhibition. Proc Natl Acad Sci U S A 106, 20127-32
19901323
Curated Info
|
|
3
|
(2009) CST Curation Set: 6682; Year: 2009; Biosample: Dana Farber BEAS LKB; Disease: -; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info
|
|
4
|
(2006) CST Curation Set: 2043; Year: 2006; Biosample: LAN-1; Disease: neuroblastoma; Treatment: serum starved; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info
|
|
5
|
Tennagels N, Bergschneider E, Al-Hasani H, Klein HW (2000) Autophosphorylation of the two C-terminal tyrosine residues Tyr1316 and Tyr1322 modulates the activity of the insulin receptor kinase in vitro. FEBS Lett 479, 67-71
10940390
Curated Info
|
|
6
|
Noelle V, Tennagels N, Klein HW (2000) A single substitution of the insulin receptor kinase inhibits serine autophosphorylation in vitro: evidence for an interaction between the C-terminus and the activation loop. Biochemistry 39, 7170-7
10852715
Curated Info
|
|
7
|
Soni P, et al. (2000) The differential effects of pp120 (Ceacam 1) on the mitogenic action of insulin and insulin-like growth factor 1 are regulated by the nonconserved tyrosine 1316 in the insulin receptor. Mol Cell Biol 20, 3896-905
10805733
Curated Info
|
|
8
|
Al-Hasani H, et al. (1997) Identification of Ser-1275 and Ser-1309 as autophosphorylation sites of the insulin receptor. FEBS Lett 400, 65-70
9000514
Curated Info
|
|
9
|
Lewis RE, Volle DJ, Sanderson SD (1994) Phorbol ester stimulates phosphorylation on serine 1327 of the human insulin receptor. J Biol Chem 269, 26259-66
7929343
Curated Info
|
|
10
|
Ando A, et al. (1992) Enhanced insulin-induced mitogenesis and mitogen-activated protein kinase activities in mutant insulin receptors with substitution of two COOH-terminal tyrosine autophosphorylation sites by phenylalanine. J Biol Chem 267, 12788-96
1618780
Curated Info
|
|
11
|
Murakami MS, Rosen OM (1991) The role of insulin receptor autophosphorylation in signal transduction. J Biol Chem 266, 22653-60
1658004
Curated Info
|
|
12
|
Baron V, et al. (1991) The carboxyl-terminal domain of the insulin receptor: its potential role in growth-promoting effects. Biochemistry 30, 9365-70
1654097
Curated Info
|
|
13
|
King MJ, Sale GJ (1990) Dephosphorylation of insulin-receptor autophosphorylation sites by particulate and soluble phosphotyrosyl-protein phosphatases. Biochem J 266, 251-9
1689998
Curated Info
|
|
14
|
Tavaré JM, Denton RM (1988) Studies on the autophosphorylation of the insulin receptor from human placenta. Analysis of the sites phosphorylated by two-dimensional peptide mapping. Biochem J 252, 607-15
3166375
Curated Info
|
