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Phosphorylation Site Page:
Tyr1355 - INSR (human)

Site Information
SLGFKRSyEEHIPYT    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: INSR iso2 (human): Y1343, INSR (mouse): Y1345, INSR (rat): Y1356
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447683

In vivo Characterization
Methods used to characterize site in vivo: 2D analysis (11), immunoprecipitation (13), mass spectrometry (1, 2, 3, 4, 5, 6), mutation of modification site (9, 11, 12, 13), phospho-antibody (9, 13, 14), phosphoamino acid analysis (11), phosphopeptide mapping (11), western blotting (9)
Disease tissue studied: neuroblastoma (1, 6)
Relevant cell line - cell type - tissue: BEAS-2B (epithelial) [LKB1 (human), no information] (5), CHO (fibroblast) (12), CHO (fibroblast) [EphB1 (human), transfection] (13), COS (fibroblast) (11), HCT116 (intestinal) (2), hepatocyte [INSR (mouse)] (9), HES-3 ('stem, embryonic') (3), KMS-11 (B lymphocyte) (4), LAN-1 (neural crest) (1, 6), MEF (fibroblast) [INSR (human), transfection] (14), SKNBE(2) (neural crest) (1)

Controlled by
Kinases, in vitro: INSR (human) (7, 8, 10, 13, 15, 16)
Phosphatases, in vitro: PTPRA (rat) (15)
Treatments: insulin (9, 11)

Downstream Regulation
Effects of modification on INSR: enzymatic activity, induced (7, 8, 9), protein conformation (7)
Effects of modification on biological processes: cell cycle regulation (12), cell growth, altered (9, 14)



Palacios-Moreno J, et al. (2015) Neuroblastoma Tyrosine Kinase Signaling Networks Involve FYN and LYN in Endosomes and Lipid Rafts. PLoS Comput Biol 11, e1004130
25884760   Curated Info


Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info


Ding VM, et al. (2011) Tyrosine phosphorylation profiling in fgf-2 stimulated human embryonic stem cells. PLoS One 6, e17538
21437283   Curated Info


St-Germain JR, et al. (2009) Multiple myeloma phosphotyrosine proteomic profile associated with FGFR3 expression, ligand activation, and drug inhibition. Proc Natl Acad Sci U S A 106, 20127-32
19901323   Curated Info


(2009) CST Curation Set: 6682; Year: 2009; Biosample/Treatment: cell line, Dana Farber BEAS LKB/untreated; Disease: -; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info


(2006) CST Curation Set: 2043; Year: 2006; Biosample/Treatment: cell line, LAN-1/serum starved; Disease: -; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info


Tennagels N, Bergschneider E, Al-Hasani H, Klein HW (2000) Autophosphorylation of the two C-terminal tyrosine residues Tyr1316 and Tyr1322 modulates the activity of the insulin receptor kinase in vitro. FEBS Lett 479, 67-71
10940390   Curated Info


Noelle V, Tennagels N, Klein HW (2000) A single substitution of the insulin receptor kinase inhibits serine autophosphorylation in vitro: evidence for an interaction between the C-terminus and the activation loop. Biochemistry 39, 7170-7
10852715   Curated Info


Soni P, et al. (2000) The differential effects of pp120 (Ceacam 1) on the mitogenic action of insulin and insulin-like growth factor 1 are regulated by the nonconserved tyrosine 1316 in the insulin receptor. Mol Cell Biol 20, 3896-905
10805733   Curated Info


Al-Hasani H, et al. (1997) Identification of Ser-1275 and Ser-1309 as autophosphorylation sites of the insulin receptor. FEBS Lett 400, 65-70
9000514   Curated Info


Lewis RE, Volle DJ, Sanderson SD (1994) Phorbol ester stimulates phosphorylation on serine 1327 of the human insulin receptor. J Biol Chem 269, 26259-66
7929343   Curated Info


Ando A, et al. (1992) Enhanced insulin-induced mitogenesis and mitogen-activated protein kinase activities in mutant insulin receptors with substitution of two COOH-terminal tyrosine autophosphorylation sites by phenylalanine. J Biol Chem 267, 12788-96
1618780   Curated Info


Murakami MS, Rosen OM (1991) The role of insulin receptor autophosphorylation in signal transduction. J Biol Chem 266, 22653-60
1658004   Curated Info


Baron V, et al. (1991) The carboxyl-terminal domain of the insulin receptor: its potential role in growth-promoting effects. Biochemistry 30, 9365-70
1654097   Curated Info


King MJ, Sale GJ (1990) Dephosphorylation of insulin-receptor autophosphorylation sites by particulate and soluble phosphotyrosyl-protein phosphatases. Biochem J 266, 251-9
1689998   Curated Info


Tavaré JM, Denton RM (1988) Studies on the autophosphorylation of the insulin receptor from human placenta. Analysis of the sites phosphorylated by two-dimensional peptide mapping. Biochem J 252, 607-15
3166375   Curated Info

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