Tyr1355
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Phosphorylation Site Page:
Tyr1355 - InsR (human)

Site Information
SLGFKRSyEEHIPYT    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: InsR iso2 (human): Y1343, InsR (mouse): Y1345, InsR (rat): Y1356
Blast this site against: NCBI  SwissProt  PDB 

In vivo Characterization
Methods used to characterize site in vivo: 2D analysis (10), immunoprecipitation (12), mass spectrometry (1, 2, 3, 5), mutation of modification site (8, 10, 11, 12), phospho-antibody (8, 12, 13), phosphoamino acid analysis (10), phosphopeptide mapping (10), western blotting (8)
Disease tissue studied: neuroblastoma (5)
Relevant cell line - cell type - tissue: CHO (fibroblast) (11), CHO (fibroblast) [EphB1 (human), transfection] (12), COS (fibroblast) (10), HCT116 (intestinal) (1), hepatocyte [InsR (mouse)] (8), HES-3 ('stem, embryonic') (2), KMS-11 (B lymphocyte) (3), LAN-1 (neural crest) (5), MEF (fibroblast) [InsR (human), transfection] (13)

Controlled by
Kinases, in vitro: InsR (human) (6, 7, 9, 12, 14, 15)
Phosphatases, in vitro: PTPRA (rat) (14)
Treatments: insulin (8, 10)

Downstream Regulation
Effects of modification on InsR: enzymatic activity, induced (6, 7, 8), protein conformation (6)
Effects of modification on biological processes: cell cycle regulation (11), cell growth, altered (8, 13)


References

1

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

2

Ding VM, et al. (2011) Tyrosine phosphorylation profiling in fgf-2 stimulated human embryonic stem cells. PLoS One 6, e17538
21437283   Curated Info

3

St-Germain JR, et al. (2009) Multiple myeloma phosphotyrosine proteomic profile associated with FGFR3 expression, ligand activation, and drug inhibition. Proc Natl Acad Sci U S A 106, 20127-32
19901323   Curated Info

4

(2009) CST Curation Set: 6682; Year: 2009; SILAC: N; Biosample/Treatment: Dana Farber BEAS LKB(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

(2006) CST Curation Set: 2043; Year: 2006; SILAC: N; Biosample/Treatment: LAN-1(cell line)/serum starved; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Tennagels N, Bergschneider E, Al-Hasani H, Klein HW (2000) Autophosphorylation of the two C-terminal tyrosine residues Tyr1316 and Tyr1322 modulates the activity of the insulin receptor kinase in vitro. FEBS Lett 479, 67-71
10940390   Curated Info

7

Noelle V, Tennagels N, Klein HW (2000) A single substitution of the insulin receptor kinase inhibits serine autophosphorylation in vitro: evidence for an interaction between the C-terminus and the activation loop. Biochemistry 39, 7170-7
10852715   Curated Info

8

Soni P, et al. (2000) The differential effects of pp120 (Ceacam 1) on the mitogenic action of insulin and insulin-like growth factor 1 are regulated by the nonconserved tyrosine 1316 in the insulin receptor. Mol Cell Biol 20, 3896-905
10805733   Curated Info

9

Al-Hasani H, et al. (1997) Identification of Ser-1275 and Ser-1309 as autophosphorylation sites of the insulin receptor. FEBS Lett 400, 65-70
9000514   Curated Info

10

Lewis RE, Volle DJ, Sanderson SD (1994) Phorbol ester stimulates phosphorylation on serine 1327 of the human insulin receptor. J Biol Chem 269, 26259-66
7929343   Curated Info

11

Ando A, et al. (1992) Enhanced insulin-induced mitogenesis and mitogen-activated protein kinase activities in mutant insulin receptors with substitution of two COOH-terminal tyrosine autophosphorylation sites by phenylalanine. J Biol Chem 267, 12788-96
1618780   Curated Info

12

Murakami MS, Rosen OM (1991) The role of insulin receptor autophosphorylation in signal transduction. J Biol Chem 266, 22653-60
1658004   Curated Info

13

Baron V, et al. (1991) The carboxyl-terminal domain of the insulin receptor: its potential role in growth-promoting effects. Biochemistry 30, 9365-70
1654097   Curated Info

14

King MJ, Sale GJ (1990) Dephosphorylation of insulin-receptor autophosphorylation sites by particulate and soluble phosphotyrosyl-protein phosphatases. Biochem J 266, 251-9
1689998   Curated Info

15

Tavaré JM, Denton RM (1988) Studies on the autophosphorylation of the insulin receptor from human placenta. Analysis of the sites phosphorylated by two-dimensional peptide mapping. Biochem J 252, 607-15
3166375   Curated Info

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